2012 Fiscal Year Final Research Report
Elucidation of mechanism for reorganization of host cytoskeletoninduced by Sendai virus matrix protein
| Project/Area Number |
23790505
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Hiroshima University |
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Principal Investigator |
IRIE Takashi 広島大学, 大学院・医歯薬保健学研究院, 准教授 (70419498)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 出芽 / 細胞骨格 / マトリクス蛋白質 |
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| Research Abstract |
In this study, we aimed at elucidation of importance of reorganization of host cellular cytoskeleton induced by SeV matrix protein M on viral assembly and budding, and we found that SeV M protein has the ability to induce reorganization of actin cytoskeleton to form filopodia-like actin-based structure in a Rho-family GTPase Cdc42-dependent manner. This actin reorganization was important for efficient budding of virus-like particles formed by expression of M alone. In addition, we also showed a mechanism for polarized regulation of viral RNA synthesis by one of the SeV accessory protein C, a potential importance of C protein on viral replication, a novel mechanism for inhibition of IFN-β induction by another SeV accessory protein V, and an importance of SeV nucleoprotein N on restriction of DI genome produc tion and evasion form detection of viral infection by host innate immune sensors.
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