Studies on the identification and acquisition mechanism ofcell-derived membrane on the surface of hepatitis E virus particles

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790508
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Jichi Medical University
• Principal Investigator: NAGASHIMA Shigeo 自治医科大学, 医学部, 講師 (60433116)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO Hiroaki 自治医科大学, 医学部, 教授 (30177092) ; TAKAHASHI Masaharu 自治医科大学, 医学部, 講師 (70326841) ; NISHIZAWA Tsutomu 自治医科大学, 医学部, 講師 (30306112) ; KOBAYASHI Tominari 自治医科大学, 医学部, 助教 (00634164)
• Research Collaborator: YASUDA Jiro 長崎大学, 熱帯医学研究所, 教授 (10282518)
• Project Period (FY): 2011 – 2012
• Keywords: E 型肝炎ウイルス / 粒子形成 / 放出 / MVB 経路 / Tsg101 / Vps4 / エンドソーム輸送

Research Abstract

The present study revealed that HEV requires the MVB pathway for release of virus particles. Furthermore, our results indicate that membrane-associated HEV particles retain the antigenicity of TGOLN2 derived from TGN on the surface of the particles

Research Products

• [Journal Article] Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia (2013)
  • Author(s): Mulyanto S, Depamede SN, Sriasih M, Takahashi M, Nagashima S, Jirintai S, Nishizawa T, Okamoto H
  • Journal Title: Arch Virol Volume: 158 Pages: 87-96
  • DOI: DOI:10.1007/s00705-012-1462-0
  • Peer Reviewed
• [Journal Article] Molecular analysis of hepatits E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells (2012)
  • Author(s): Jirintai S, Jinshan, Tanggis, Manglai D, Mulyanto, Takahashi M, Nagashima S, Kobayashi T, Nishizawa T, Okamoto H
  • Journal Title: Virus Res Volume: 170 Pages: 126-137
  • DOI: DOI:10.1016/j.virusres.2012.09.015
  • Peer Reviewed
• [Journal Article] A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food (2012)
  • Author(s): Takahashi H, Tanaka T, Jirintai S,Nagashima S, Takahashi M, Nishizawa T, Mizuo H, Yazaki Y, Okamoto H
  • Journal Title: Arch Virol Volume: 157(2) Pages: 235-46
  • DOI: DOI:10.1007/s00705-011-1153-2
  • Peer Reviewed
• [Journal Article] Tumour susceptibility gene 101 and the vacuolar protein sorting pathway are required for the release of hepatitis E virions (2011)
  • Author(s): Nagashima S, Takahashi M, Jirintai S, Tanaka T, Nishizawa T, Yasuda J, Okamoto H
  • Journal Title: J Gen Virol Volume: 92(Pt 12) Pages: 2838-48
  • DOI: DOI:10.1099/vir.0.035378-0
  • Peer Reviewed
• [Presentation] 培養細胞から放出された E 型肝炎ウイルスの粒子表面に存在する膜成分の抗原性の解析 (2012)
  • Author(s): 長嶋茂雄、高橋雅春、吉林台、西澤勉、小林富成、岡本宏明
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-14
• [Presentation] Tsg101 and the vacuolar protein sorting pathway are required for virion release of hepatitis E virus (2011)
  • Author(s): Nagashima S, Takahashi M, Jirintai, Tanaka T, Nishizawa T, Yasuda J, Okamoto H
  • Organizer: 15thInternational Congress of Virology (第59回日本ウイルス学会学術集会)
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Remarks] 自治医科大学 医学部 感染・免疫学講座ウイルス学部門
  • URL: http://www.jichi.ac.jp/virology/