2013 Fiscal Year Final Research Report
Mechanistic analysis of hepatitis C virus life cycle using small molecules
| Project/Area Number |
23790514
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
WATASHI Koichi 国立感染症研究所, ウイルス第二部, 主任研究官 (40378948)
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| Project Period (FY) |
2011 – 2012
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| Keywords | HCV / PLD / 抗ウイルス |
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| Research Abstract |
We analyzed the anti-hepatitis C virus (HCV) mechanism of halopemide, which was identified to reduce HCV production by a cell-based chemical screening. Halopemide inhibited the secretion process of HCV. Its anti-HCV effect was mediated by host phospholipase D (PLD). PLD regulated the release of HCV but not host secreted proteins. Inhibition of PLD stalled HCV particles around the Golgi. The above data suggest that host PLD specifically mediates the post-Golgi trafficking of HCV, and halopemide inhibited this process to reduce the production of infectious HCV.
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