Determination of the role of anti-flavivirus antibody in antibody-dependent enhancement infection of dengue virus

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790515
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MOI Meng ling 国立感染症研究所, ウイルス第一部, 研究員 (40597499)
• Project Period (FY): 2011 – 2012
• Keywords: 感染防御・抑制 / 診断・治療 / 感染免疫

Research Abstract

In this study the role of anti-flavivirus antibodies during antibody-dependent enhancement of dengue virus (DENV) infection was characterized by using FcγR-expressing cells. Infection-enhancement antibody enhances dengue virus (DENV) replication in FcγR-expressing cells, contributing to high levels of viral replication during secondary infection; a prelude of severe DENV illness. The neutralizing and infection-enhancement activity of antibodies after dengue virus (DENV) infection, Japanese encephalitis virus (JEV) infection and JE vaccination was determined in this study. Using FcγR-expressing cells, serum samples from the early phase of DENV infection exhibited ADE activity but serum samples from late phase of DENV infection exhibited DENV neutralizing activity. Serum sample from a JEV patient exhibited ADE activity during the late phase of JEV infection. Additionally, serum samples from JEV vaccinees exhibited DENV cross-reactive IgG antibodies after JEV vaccination, but neutralizing activity to DENV was absent. The results suggest that other than DENV, flavivirus infection and vaccination also induce cross-reactive antibodies to DENV but cross-neutralizing activity may be low or absent, implying a role of these anti-flavivirus antibodies during DENV infection.

Research Products

• [Journal Article] Dengue virus type-3 infection in a traveler returning from Benin to Japan. (2012)
  • Author(s): Ujiie M, Moi ML, Kobayashi T, Takeshita N, Kato Y, Takasaki T, Kanagawa S
  • Journal Title: Journal of Travel Medicine, Volume: 19,4 Pages: 255-257
• [Journal Article] Changes in hematological and serum biochemical parameters in common marmosets (Callithrix jacchus) after inoculation with dengue virus. (2012)
  • Author(s): Omatsu T, Moi ML, Takasaki T, Nakamura S, Katakai Y, Tajima S, Ito M, Yoshida T, Saito A, Akari H, Kurane I
  • Journal Title: Journal of Medical Primatology Volume: 41,5 Pages: 289-296
• [Journal Article] Dengue virus infection-enhancing activity in serum samples with neutralizing activity as determined by using FcγR-expressing cells (2012)
  • Author(s): Moi ML, Lim CK, Chua KB, Takasaki T, Kurane I
  • Journal Title: PLoS Neglected Tropical Diseases Volume: 6(2) Pages: e1536
• [Journal Article] Demographic features of imported dengue fever and dengue haemorrhagic fever in Japan from 2006 to 2009 (2012)
  • Author(s): Takasaki T, Kotaki A, Tajima S, Omatsu T, Harada F, Lim CK, Moi ML, Ito M, Ikeda M, Kurane I
  • Journal Title: Dengue Bulletin Volume: 35 Pages: 217-222
• [Journal Article] 感染症迅速診断キットの有用性と限界:デング熱 (2012)
  • Author(s): Moi ML, 高崎智彦
  • Journal Title: 小児科 Volume: 53(4) Pages: 457-465
• [Journal Article] クロアチアにおけるデング熱の流行 (2011)
  • Author(s): Moi ML
  • Journal Title: Infectious Agents Surveillance Report Volume: 32 (6) Pages: 165-167
• [Journal Article] アフリカからのデング熱輸入例 (2011)
  • Author(s): Moi ML, Takasaki T, Iwagoe H, Sakamoto M, Kobayashi K, Ujiie M.
  • Journal Title: Infectious Agents Surveillance Report Volume: 32 (6) Pages: 164-165
• [Journal Article] Detection of higher levels of dengue viremia using FcγR-expressing BHK-21 cells than FcγR negative cells in secondary infection but not in primary infection. (2011)
  • Author(s): Moi ML, Lim CK, Kotaki A, Takasaki T, Kurane I.
  • Journal Title: Journal of Infectious Diseases Volume: 203(10) Pages: 1405-1414
• [Journal Article] Dengue virus isolation relying on antibody-dependent enhancement mechanism using FcγR-expressing BHK cells and a monoclonal antibody with infection-enhancing capacity. (2011)
  • Author(s): Moi ML, Lim CK, Tajima S, Kotaki A, Saijo M, Takasaki T, Kurane I.
  • Journal Title: Journal of Clinical Virology. Volume: 52(3) Pages: 225-230
• [Journal Article] Dengue maculopathy in a traveler (2011)
  • Author(s): Ujiie M, Moi ML, Takeda N.
  • Journal Title: American Journal of Tropical Medicine and Hygiene. Volume: 85(6) Pages: 965-966
• [Journal Article] Common marmoset (Callithrix jacchus) as a primate model of dengue virus infection: development of high levels of viremia and demonstration of protective immunity. (2011)
  • Author(s): Omatsu T, Moi ML, Hirayama T, Takasaki T, Nakamura S, Tajima S, Ito M, Yoshida T, Saito A, Katakai Y, Akari H, Kurane I.
  • Journal Title: Journal of General Virology. Volume: 92 Pages: 2272-2280
• [Presentation] Re-assessment of dengue neutralizing antibody and viremia titers in dengue patients using FcγR-expressing cells (2012)
  • Author(s): Moi ML, Lim CK, Saijo M, Takasaki T, Kurane I.
  • Organizer: The 61st Annual Meeting of the American Society of Tropical Medicine and Hygiene
  • Place of Presentation: Atlanta, USA
  • Year and Date: 20121100
• [Presentation] Dengue fever outbreak among Japanese construction workers returning from India (2012)
  • Author(s): Ujiie M, Moi ML, Kato Y, Kotaki A, Takeshita N, Kanagawa S, Takasaki T, Ohmagari N.
  • Organizer: The 61st Annual Meeting of the American Society of Tropical Medicine and Hygiene
  • Place of Presentation: Atlanta, USA
  • Year and Date: 20121100
• [Presentation] Role of antibodies in dengue infection and protective immunity during secondary infection of marmosets (2012)
  • Author(s): Moi ML, Omatsu T, Nakamura S, Takasaki T, Ami Y, Katakai Y, Suzaki Y, Akari H, Kurane I.
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20121100
• [Presentation] ウイルスRNA安定保存キットの評価, (2012)
  • Author(s): 小滝徹、モイメンリン、田島茂、高崎智彦.
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20121100
• [Presentation] マーモセットを用いたチクングンニヤウイルスの霊長類モデルの検討 (2012)
  • Author(s): 林昌宏、網康至、藤井克樹、北浦一孝、モイメンリン、白井顕治、小滝徹、須崎百合子、森川茂、西條政幸、鈴木隆二、倉根一郎、高崎智彦.
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20121100
• [Presentation] Dengue vaccine development: re-assessment fo dengue neutralizing antibody and viremia titers using FcγR-expressing cells (2012)
  • Author(s): Moi ML, Lim CK, Saijo M, Takasaki T, Kurane I
  • Organizer: The 34th Naito Conference: Infection, immunity and their control for health
  • Place of Presentation: Hokkaido
  • Year and Date: 20121000
• [Presentation] Serological evidence of the co-circulation of multiple dengue virus serotypes in Kuala Lumpur, Malaysia (2012)
  • Author(s): Azami NAM, Moi ML, Takasaki T, Salleh SA, Neoh HM, Othman Z, Shah SA, Kurane I, Jamal R.
  • Organizer: 13^<th> Asia-Pacific Congress on Clinical Microbiology and Infection.
  • Place of Presentation: Beijing, China
  • Year and Date: 20121000
• [Presentation] Ocular complications associated with imported dengue fever (2012)
  • Author(s): Ujiie M, Moi ML, Kato Y, Kanagawa S, Ohmagari N, Takeshita N, Takasaki T
  • Organizer: 9 ^<th> Asia Pacific Travel Health Conference in conjunction with the 5^<th> Regional Meeting of the International Society of Travel Medicine.
  • Place of Presentation: Singapore
  • Year and Date: 20120500
• [Presentation] Application of the dengue non-structural protein 1 (NS1) ELISA for the detection of dengue virus infection in travelers (2012)
  • Author(s): Moi ML, Omatsu T, Tajima S, Lim CK, Saijo M, Kurane I, Takasaki T.
  • Organizer: 9 ^<th> Asia Pacific Travel Health Conference in conjunction with the 5^<th> Regional Meeting of the International Society of Travel Medicine.
  • Place of Presentation: SingaporeMoi ML, Lim CK, Saijo M, Kurane I, Takasaki T
  • Year and Date: 20120500
• [Presentation] デング熱診断サーベイランスのためのNS1抗原検出診断キット (2012)
  • Author(s): Moi ML, Lim CK, Saijo M, Kurane I, Takasaki T.
  • Organizer: 86^<th> Annual Meeting of the Japanese Association for Infectious Diseases
  • Place of Presentation: Nagasaki, Japan
  • Year and Date: 20120400
• [Presentation] Molecular diagnosis and analysis of imported Chikungunya virus strains, Japan, 2006-2010 (2011)
  • Author(s): Lim CK, Takasaki T, Moi ML, Kotaki A, Kurane I, Saijo M.
  • Organizer: The 60th Annual Meeting of the American Society for Tropical Medicine and Hygiene,
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 20111200
• [Presentation] Detection of higher levels of dengue viremia using FcγR-expressing BHK-21 cells than FcγR-negative cells in secondary infection but not in primary infection (2011)
  • Author(s): Moi ML, Lim CK, Kotaki A, Takasaki T, Kurane I.
  • Organizer: International Congress of Virology
  • Place of Presentation: 北海道
  • Year and Date: 20110900
• [Presentation] Pathogenesis of epidemic chikungunya virus in nonhuman primates (2011)
  • Author(s): Lim CK, Ami Y, Fujii Y, Moi ML, Kitaura K, Kotaki A, Morikawa S, Saijo M, Suzuki R, Kurane I, Takasaki T
  • Organizer: International Congress of Virology
  • Place of Presentation: 北海道
  • Year and Date: 20110900
• [Presentation] Antibody-dependent enhancement of dengue virus infection: revisit of antibody response and viremia in dengue patients using FcγR-expressing cells (2011)
  • Author(s): Moi ML, Lim CK, Ito M, Takasaki T, Kurane I.
  • Organizer: 45th Joint Working Conference on Immunology and Viral Diseases, US-Japan Cooperative Medical Science Program.
  • Place of Presentation: Stanford University, カリフォルニア, 米国
  • Year and Date: 20110600
• [Book] 国内発生はほとんどないが注目される感染症1-デング熱・出血熱. 小児感染症学 (2011)
  • Author(s): Moi ML, 高崎智彦
  • Total Pages: 508-511
• [Remarks]
  • URL: http://www0.nih.go.jp/vir1/NVL/papers_web/papers_vbv.htm
• [Remarks]
  • URL: https://sites.google.com/site/sherrymoi/