The role for Laptm5 in the down-modulation of pre-B cell receptor

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790530
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KAWANO Youhei 東京医科歯科大学, 大学院・医歯学・総合研究科, 助教 (20401383)
• Project Period (FY): 2011 – 2012
• Keywords: プレB細胞 / pre-BCR

Research Abstract

Pre-B cell receptor (pre-BCR) expression has to be strictly regulated for normal B cell development, and its dysregulation is associated with anomalies of B-lineage cells, including leukemogenesis. However, it remained elusive on the mechanism underlying pre-BCR down-modulation although transcriptional silencing of pre-BCR component is currently proposed. In this paper, we found that a lysosomal membrane protein, Laptm5 is up-regulated via autonomous pre-BCR signaling, followed by pre-BCR down-modulation by promoting its degradation in lysosomes. Of note, this finding suggests there is another mechanism of pre-BCR down-modulation at the protein level through the regulation of Laptm5.

Research Products

• [Journal Article] A novel mechanism for the autonomous termination of pre-B cell receptor expression via induction of lysosomal-associated protein transmembrane 5. (2012)
  • Author(s): Kawano, Y., Ouchida, R., Wang
  • Journal Title: Mol. Cell. Biol. Volume: 32 Pages: 4462-71
• [Presentation] A novel mechanism for the autonomous termination of pre-B cell receptor expression via induction of lysosome-associated protein transmembrane 5 (2013)
  • Author(s): Kawano, Y., Ouchida, R., O-Wang, J., Yoshikawa, S., Yamamoto, M., Kitamura, D., Karasuyama, H.
  • Organizer: Keystone Symposia-B cell development and function
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 20130210-15
• [Presentation] A novel mechanism for the autonomous termination of pre-B cell receptor expression via induction of lysosome-associated proteintransmembrane 5 (2012)
  • Author(s): Kawano, Y., Ouchida, R., O-Wang, J., Yoshikawa, S., Yamamoto, M., Kitamura, D., Karasuyama, H.
  • Organizer: The 35th MBSJ annualmeeting.
  • Place of Presentation: Fukuoka
  • Year and Date: 20121210-14
• [Presentation] Laptm5 down-modulates pre-B cell receptor in anautophagy-independent manner (2011)
  • Author(s): Kawano, Y., Ouchida, R., O-Wang, J., Yoshikawa, S., Yamamoto, M., Kitamura, D., Karasuyama, H.
  • Organizer: The 41th JSI annual meeting.
  • Place of Presentation: Chiba
  • Year and Date: 20111127-29