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2012 Fiscal Year Final Research Report

Suppression of autoimmunity by B lymphocyte membrane protein CD72

Research Project

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Project/Area Number 23790531
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

WATANABE Kozo  東京医科歯科大学, 難治疾患研究所, 助教 (20376718)

Project Period (FY) 2011 – 2012
KeywordsBリンパ球 / レクチン / 糖鎖
Research Abstract

C-type lectin-like domain (CTLD) of B cell membrane protein CD72 is suggested to associated with development of autoimmune diseases. However, the glycan ligand and function of its CTLD is not clear. In this study, some kinds of sulfated glycans were identified as CD72 ligands by glycan array screening using CD72 recombinant protein.Interestingly, some of the identified CD72 ligands are autoimmune disease-associated antigens. In addition, the effect of CD72 ligand on B cell antigen receptor (BCR) signaling in primary B cells from QM mice and QM CD72 deficient mice by analyzing calcium influx using NP-conjugated CD72 ligand and NP-conjugated BSA, showed CD72-dependent reduction of BCR signaling following ligation of CD72 ligand-containing antigen. Moreover, the amount of serum anti-CD72 ligand IgM classantibody was increased in C57BL/6 CD72 deficient mice compared with that in C57BL/6 mice. These results suggest novel self and non-self recognition mechanism of B cells by CD72-CD72 ligand interaction-mediated suppression of BCR signaling and antibody production against CD72 ligand-containing antigens.

  • Research Products

    (3 results)

All 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results)

  • [Journal Article] The use of cationic nanogels to deliver proteins to myeloma cells and primary T lymphocytes that poorly express heparan sulfate.2011

    • Author(s)
      Watanabe, K., Tsuchiya, Y., Kawaguchi, Y., Sawada, S., Ayame, H., Akiyoshi, K. and Tsubata, T.
    • Journal Title

      Biomaterials.

      Volume: 32 Pages: 5900-5905

    • Peer Reviewed
  • [Journal Article] CD22-antagonists with nanomolar potency: The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold.2011

    • Author(s)
      Abdu-Allah, H. H. M., Watanabe, K., Completo, G. C., Sadagopan, M., Hayashizaki, K., Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Paulson, J.C., Tsubata, T., Ando, H., Ishida, H. and Kiso, M.
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 19 Pages: 1966-1971

    • Peer Reviewed
  • [Journal Article] Design and synthesis of multivalent heterobifunctional CD22-ligand as a potential immunomodulator.2011

    • Author(s)
      Abdu-Allah, H. H. M., Watanabe, K., Kanie, O., Tsubata, T. Ishida, H. and Kiso, M.
    • Journal Title

      Synthesis.

      Volume: 18 Pages: 2968-2974

    • Peer Reviewed

URL: 

Published: 2014-09-25  

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