2012 Fiscal Year Final Research Report
Development of a "new generation" monitoring system of passive smoking based on antibody engineering approach
| Project/Area Number |
23790632
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
MORITA Izumi 神戸薬科大学, 薬学部, 助手 (20299085)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 抗体工学 / 受動喫煙 / バイオテクノロジー |
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| Research Abstract |
We previously established a hybridoma clone which produces an anti-cotinine antibody and "wild type" scFv is prepared by combining the V_H and V_L domains of theantibody. Using this scFv gene as a template, a variety of scFv gene library is produced, from which ascFv clones with a higher affinity were obtained. The conditions of competitive ELISA using thesescFvs are optimized, and consequently, the assay sensitivity was largely improved compared with theconventional ELISA using a mouse antibody. This assay system was applicable to the determination ofhuman urinary cotinine levels, and is expected to be useful for monitoring of passive smoking.
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