Analysis of gene expression regulation of intelectin-1 in malignant pleural mesothelioma

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790634
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kanagawa Cancer Center Research Institute
• Principal Investigator: YAMASHITA Makiko (辻 真紀子) 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, 客員研究員 (60397776)
• Research Collaborator: TSUJI Shoutaro 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がん治療学部, 主任研究員 (30285192)
• Project Period (FY): 2011 – 2013
• Keywords: 中皮腫

Research Abstract

In this study, we found that intelectin-1 was expressed in malignant pleural mesothelioma (MPM) or intestinal metaplasia in stomach, but not in normal pleura and normal stomach. However, expression of other lesion markers was not similar. Therefore, intelectin-1 expression in MPM and intestinal metaplasia may be regulated by different gene regulatory mechanisms each other. The promoter and enhancer of intelectin-1 in MPM located on a region from -56 to +6 nucleotides including a TATA box element and from +263 to +293 nucleotides, respectively, for the transcription start site. Database analysis of the enhancer region suggested that Oct and Sox transcriptional factors might function as transcriptional factors of intelectin-1 in MPM.

Research Products

• [Journal Article] Hishot display-a new combinatorial display for obtaining target-recognizing peptides (2013)
  • Author(s): Shoutaro Tsuji, Makiko Yamashita, Taihei Kageyama, Takashi Ohtsu, Katsuo Suzuki, Shintaro Kato, Joe Akitomi, Makio Furuichi, and Iwao Waga
  • Journal Title: PLoS ONE Volume: 8(12) Pages: e83108
  • DOI: 10.1371/journal.pone.0083108
  • Peer Reviewed
• [Journal Article] Specific expression of human intelectin-1 in malignant pleural mesothelioma and gastrointestinal goblet cells (2012)
  • Author(s): Kota Washimi, Tomoyuki Yokose, Makiko Yamashita, Taihei Kageyama, Katsuo Suzuki, Mitsuyo Yoshihara, Yohei Miyagi, Hiroyuki Hayashi, and Shoutaro Tsuji
  • Journal Title: PLoS ONE Volume: 7(7) Pages: e39889
  • DOI: 10.1371/journal.pone.0039889
  • Peer Reviewed
• [Presentation] Hishot display - a new combinatorial display for obtaining artificial antibody (2013)
  • Author(s): Shoutaro Tsuji, Makiko Yamashita, Taihei Kageyama, Takashi Ohtsu, Katsuo Suzuki, Shintaro Kato, Joe Akitomi, Makio Furuichi, Iwao Waga
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2013-12-03
• [Presentation] 上皮型中皮腫におけるインテレクチン-1の発現と診断マーカーとしての評価 (2013)
  • Author(s): 辻祥太郎、影山泰平、鷲見公太、山下真紀子、横瀬智之、諸星隆夫、津浦幸夫
  • Organizer: 第4回Japan Mesothelioma Interest Group研究会
  • Place of Presentation: 京都私学会館(京都)
  • Year and Date: 2013-08-31
• [Presentation] インテレクチン-1は上皮型悪性胸膜中皮腫の優れた診断マーカーである (2012)
  • Author(s): Shoutaro Tsuji, Kota Washimi, Makiko Yamashita, Taihei Kageyama, Katsuo Suzuki, Mitsuyo Yoshihara, Yohei Miyagi
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
  • Year and Date: 2012-09-21
• [Presentation] 悪性胸膜中皮腫と腸上皮化生におけるマーカー蛋白質の発現の比較 (2012)
  • Author(s): Makiko Yamashita, Kota Washimi, Taihei Kageyama, Katsuo Suzuki, Mitsuyo Yoshihara, Tomoyuki Yokose, Yohei Miyagi, Shoutaro Tsuji
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
  • Year and Date: 2012-09-21
• [Presentation] 悪性胸膜中皮腫に対するモノクローナル抗体の作製 (2012)
  • Author(s): Taihei Kageyama, Makiko Yamashita, Katsuo Suzuki, Mitsuyo Yoshihara, Kota Washimi, Tomoyuki Yokose, Yohei Miyagi, Shoutaro Tsuji
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
  • Year and Date: 2012-09-21