2012 Fiscal Year Final Research Report
Generation of human P450 medaka for assessment of chemicals.
| Project/Area Number |
23790673
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | CYP1A / メダカ / 多環芳香族炭化水素 |
|---|
| Research Abstract |
We generated the medaka lacking cyp1a gene by TILLING. RT-PCR, Western blot analysis, and EROD (ethoxyresorufin-O-deethylase) assay revealed that the CYP1A activity was completely abrogated in these mutants. The cyp1a-deficient medaka died when exposed
|
