2012 Fiscal Year Final Research Report
The possibility of unknown viral infection with primary biliary cirrhosis and the disease association with anellovirus infection.
| Project/Area Number |
23790765
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 肝臓学 |
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| Research Abstract |
We tried to sequence hepatitis C virus (HCV) genome with RDV method, before conducting RDV method with serum in PBC patients or NOD. c3c4 mice. But this method seemed to be impossible to detect unknown viral sequence. Therefore, we tried to use deep sequencing technology.
Result: The reads of HCV origin mapped 8583 reads on the reference HCV genome. The reads of HCV origin were detectable in about 0.10% of the total reads. Surprisingly, the coverage showed 98.8% and read depth indicated 69.5x. In NS3, the position of 5 amino acid bases showed mixed variants.
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Research Products
(11 results)
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[Journal Article] Use of Illumina deep sequencing technology to differentiate hepatitis C virus variants2012
Author(s)
Ninomiya M, Ueno Y, Funayama R, Nagashima T, Nishida Y, Kondo Y, Inoue J, Kakazu E, Kimura O, Nakayama K, Shimosegawa T
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Journal Title
Journal of Clinical Microbiology
Volume: 50(3)
DOI
Peer Reviewed
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