2012 Fiscal Year Final Research Report
Analysis of intestinal mucosal repair by Notch-activated stem cells
| Project/Area Number |
23790777
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OKAMOTO Ryuichi 東京医科歯科大学, 医歯学総合研究科, 准教授 (50451935)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 炎症性腸疾患 / Notchシグナル / 腸管上皮 / 上皮幹細胞 / 粘膜再生 |
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| Research Abstract |
Through our studies aimed to develop a primary culture system of the intestinal epithelial cells, in which Notch-activated stem cell can be easily visualized, we succeeded to establish a culture method using the LGR5-EGFP mice. Using those mice, we found that the intestinal organoids that were maintained in our culture method contains high number of stem cells. In addition, transplantation of those organiods to a colitic mice model resulted in re-construction of the damaged epithelia by the organoids, which was maintained for a sustained time period. These results establish the basis for cell transplantation-mediated regeneration therapy for intestinal diseases.
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Research Products
(32 results)
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[Journal Article] The acquisition of malignant potential in colon cancer is regulated by the stabilization of Atonal homolog 1 protein.2013
Author(s)
Kano Y, Tsuchiya K, Zheng X, Horita N, Fukushima K, Hibiya S, Yamauchi Y. Nishimura T. Hinohara K, Gotoh N, Suzuki S, Okamoto R, Nakamura T, Watanabe M.
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Journal Title
Biochem Biophys Res Commun.
Volume: 432
Pages: 175-181
DOI
Peer Reviewed
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[Journal Article] Wnt5 signaling mediates biliary differentiation of fetal hepatic stem/progenitor cells.2013
Author(s)
Kiyohashi K, Kakinuma S, Kamiya A, Sakamoto N, Nitta S, Yamanaka H, Yoshino K, Fujiki J, Murakawa M, Kusano-Kitazume A, Shimizu H, Okamoto R, Azuma S, Nakagawa M, Asahina Y, Tanimizu N, Kikuchi A, Nakauchi H, Watanabe M.
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Journal Title
Hepatology
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] Real-time analysis ofP-glycoprotein-mediated drug transport across primary intestinal epithelium three-dimensionally cultured in vitro.2012
Author(s)
Mizutani T, Nakamura T, Morikawa R, Fukuda M, Mochizuki W, Yamauchi Y, Nozaki K, Yui S, Nemoto Y, Nagaishi T, Okamoto R, Tsuchiya K, Watanabe M.
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Journal Title
Biochem Biophys Res Commun
Volume: 419
Pages: 238-243
DOI
Peer Reviewed
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[Journal Article] The development of colitogenic CD4+ T cells is regulated by IL-7 in collaboration with NK cell function in a murine model of colitis.2012
Author(s)
Yamaji O, Nagaishi T, Totsuka T, Onizawa M, Suzuki M, Tsuge N, Hasegawa A, Okamoto R, Tsuchiya K, Nakamura T, Arase H, Kanai T, Watanabe M.
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Journal Title
J Immunol.
Volume: 188(6)
Pages: 2524-2536
DOI
Peer Reviewed
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[Journal Article] Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell.2012
Author(s)
Yui S, Nakamura T, Sato T, Nemoto Y, Mizutani T, Zheng X, Ichinose S, Nagaishi T, Okamoto R, Tsuchiya K, Clevers H, Watanabe M.
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Journal Title
Nat Med.
Volume: 18
Pages: 618-623
DOI
Peer Reviewed
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[Journal Article] Longitudinal cell formation in the entire human small intestine is correlated with the localization of Hath1 and Klf4.2011
Author(s)
Iwasaki M, Tsuchiya K, Okamoto R, Zheng X, Kano Y, Okamoto E, Okada E, Araki A, Suzuki S, Sakamoto N, Kitagaki K, Akashi T, Eishi Y, Nakamura T, Watanabe M.
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Journal Title
J Gastroenterol.
Volume: 46
Pages: 191-202
DOI
Peer Reviewed
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[Presentation] Regeneration of damaged colon epithelium by transplanted colon Lgr5+ stem cells maintained and expanded in vitro2012
Author(s)
Yui S, Nakamura T, Nemoto Y, Mizutani T, Fukuda M, Nozaki K, Yamauchi Y, Mochizuki W, Zheng X, Nagaishi T, Okamoto R, Tsuchiya K, Watanabe M
Organizer
第10回幹細胞シンポジウム.
Place of Presentation
淡路島
Year and Date
2012-06-01
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[Presentation] Real-time analysis of p-glycoprotein-mediated drugtransport across primary intestinal epithelial cellsthree-dimensionally cultured in vitro2012
Author(s)
Mizutani T, Nakamura T, Morikawa R, Fukuda M, Mochizuki W, Yamauchi Y, Nozaki K, Yui S, Okamoto R, Tsuchiya K, Watanabe M
Organizer
DDW2012.
Place of Presentation
San Diego
Year and Date
2012-05-21
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[Book] PD-3確定診断困難な多発小腸潰瘍の1例栄養-評価と治療2012
Author(s)
森尾純子,荒木昭博,岡田英理子,鈴木伸治,土屋輝一郎,藤井俊光,長沼誠,長堀正和,江頭徹哉,齊藤詠子,福島啓太,河合富貴子,藤木純子,櫻井幸,根本泰宏,中川美奈,岡本隆一,東正新,柿沼晴,永石宇司,中村哲也,坂本直哉,渡辺守,河内洋,江石義信
Total Pages
76-77
Publisher
メディカルレビュー社
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