Novel type IV secretion system of Helicobacter pylori

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790798
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Oita University
• Principal Investigator: SHIOTA Seiji 大分大学, 医学部, 助教 (00420646)
• Project Period (FY): 2011 – 2012
• Keywords: ヘリコバクター・ピロリ / dupA

Research Abstract

Helicobacter pylori dupA can be divided into two types according to the presence or absence of the mutation. In addition, dupA has two types with different lengths depend on the presence of approximately 600 bp in the putative 5' region (presence; long-type and absence; short-type). dupA was predicted to form a type IV secretary system (T4SS) with vir genes around dupA (dupA cluster). We reported that intact long-type dupA is a real virulence marker for severe outcomes. In addition, the presence of a complete dupAcluster (dupA and all adjacent vir genes) was associated with duodenal ulcer development.

Research Products

• [Journal Article] The significance of virulence factors inHelicobacter pylori (2013)
  • Author(s): Shiota S, Suzuki R, Yamaoka Y
  • Journal Title: J Dig Dis
  • DOI: DOI:10.1111/1751-2980.12054.
  • Peer Reviewed
• [Journal Article] The Incidence of Primary Antibiotic Resistance of Helicobacter pylori in Vietnam (2013)
  • Author(s): Binh TT, Shiota S, Nguyen LT, Ho DD, Hoang HH, Ta L, Trinh DT, Fujioka T,Yamaoka Y
  • Journal Title: J Clin Gastroenterol Volume: 47(3) Pages: 233-8
  • DOI: DOI:10.1097/MCG.0b013e3182676e2b
  • Peer Reviewed
• [Journal Article] Helicobacter pylori cagA 12-bp insertion can be a marker for duodenal ulcer in Okinawa, Japan (2013)
  • Author(s): Matsuo Y, Shiota S, Matsunari O, Suzuki R, Watada M, Binh TT, Kinjo N, Kinjo F, Yamaoka Y
  • Journal Title: J Gastroenterol Hepatol Volume: 28(2) Pages: 291-6
  • DOI: DOI:10.1111/jgh.12067.
  • Peer Reviewed
• [Journal Article] Intact Long-Type dupA as a Marker for Gastroduodenal Diseases in Okinawan Subpopulation, Japan (2013)
  • Author(s): Takahashi A, Shiota S, Matsunari O, Watada M, Suzuki R, Nakachi S, Kinjo N, Kinjo F, Yamaoka Y
  • Journal Title: Helicobacter Volume: 18(1) Pages: 66-72
  • DOI: DOI:10.1111/j.1523-5378.2012.00994.x.
  • Peer Reviewed
• [Journal Article] Helicobacter pylori infection in Japan (2013)
  • Author(s): Shiota S, Murakawi K, Suzuki R, Fujioka T, Yamaoka Y
  • Journal Title: Expert Rev Gastroenterol Hepatol Volume: 7(1) Pages: 35-40
  • DOI: DOI:10.1586/egh.12.67
  • Peer Reviewed
• [Journal Article] Association of Helicobacter pylori dupA With the Failure of Primary Eradication (2012)
  • Author(s): Shiota S, Nguyen LT, Murakami K, Kuroda A, Mizukami K, Okimoto T, Kodama M,Fujioka T, Yamaoka Y
  • Journal Title: J Clin Gastroenterol Volume: 46(4) Pages: 297-301
  • DOI: DOI:10.1097/MCG.0b013e318243201c.
  • Peer Reviewed
• [Journal Article] Helicobacter pylori iceA, Clinical Outcomes, and Correlation with cagA: A Meta-Analysis (2012)
  • Author(s): Shiota S, Watada M, Matsunari O, Iwatani S, Suzuki R, Yamaoka Y
  • Journal Title: PLoS One Volume: 7(1) Pages: 30354
  • DOI: DOI:10.1371/journal.pone.0030354
  • Peer Reviewed
• [Journal Article] Ten-year prospective follow-up of histological changes at five points on the gastric mucosa as recommended by the updated Sydney system after Helicobacter pylori eradication (2012)
  • Author(s): Kodama M, Murakami K, Okimoto T, Sato R, Uchida M, Abe T, Shiota S, Nakagawa Y, Mizukami K, Fujioka T
  • Journal Title: J Gastroenterol Volume: 47(4) Pages: 394-403
  • DOI: DOI:10.1007/s00535-011-0504-9
  • Peer Reviewed
• [Journal Article] Molecular epidemiology, population genetics, and pathogenic role of Helicobacter pylori (2012)
  • Author(s): Suzuki R, Shiota S, Yamaoka Y
  • Journal Title: Infect Genet Evol Volume: 12(2) Pages: 203-13
  • DOI: DOI:10.1016/j.meegid.2011.12.002.
  • Peer Reviewed
• [Journal Article] Association between Helicobacter pylori virulence factors andgastroduodenal diseases in Okinawa, southwestern island of Japan (2012)
  • Author(s): Matsunari O, Shiota S, Suzuki R, Watada M, Kinjo N, Murakami K, Fujioka T, Kinjo F, Yamaoka Y
  • Journal Title: J Clin Microbiol Volume: 50(3) Pages: 876-83
  • DOI: DOI:10.1128/JCM.05562-11
  • Peer Reviewed
• [Journal Article] Virulence factors or ancestral origin of Helicobacter pylori: Which is a better predictor of gastric cancer risk (2012)
  • Author(s): Shiota S, Matsunari O, Watada M, Yamaoka Y
  • Journal Title: Gut Volume: 61(3) Pages: 469-70
  • DOI: DOI:10.1136/gutjnl-2011-300317
  • Peer Reviewed
• [Journal Article] The intact dupA cluster is a more reliable Helicobacter pylori virulence marker than dupA alone (2012)
  • Author(s): Jung SW, Sugimoto M, Shiota S, Graham DY, Yamaoka Y
  • Journal Title: Infect Immun Volume: 80(1) Pages: 381-7
  • DOI: DOI:10.1128/IAI.05472-11
  • Peer Reviewed
• [Journal Article] Association between Helicobacter pylori cagA-related genes and clinical outcomes in Colombia and Japan (2011)
  • Author(s): Watada M, Shiota S, Matsunari O, Suzuki R, Murakami K, Fujioka T, Yamaoka Y
  • Journal Title: BMC Gastroenterol Volume: 11 Pages: 141
  • DOI: DOI:10.1186/1471-230X-11-141
  • Peer Reviewed
• [Journal Article] Epidemiology of Helicobacter pyloriInfection and Public Health Implications (2011)
  • Author(s): Goh KL, Chan WK, Shiota S, Yamaoka Y
  • Journal Title: Helicobacter Volume: 16 Suppl 1 Pages: 1-9
  • DOI: DOI:10.1111/j.1523-5378.2011.00874.x.
  • Peer Reviewed
• [Journal Article] Prevalence of two homologous genes encoding glycosyltransferases of Helicobacter pylori in the United States and Japan (2011)
  • Author(s): Matsuda M, Shiota S, Matsunari O, Watada M, Murakami K, Fujioka T, Yamaoka Y
  • Journal Title: J Gastroenterol Hepatol Volume: 26(9) Pages: 1451-6
  • DOI: DOI:10.1111/j.1440-1746.2011.06779.x.
  • Peer Reviewed
• [Journal Article] The relationship between Helicobacter pylori infection and Alzheimer's disease in Japan (2011)
  • Author(s): Shiota S, Murakami K, Yoshiiwa A, Yamamoto K, Ohno S, Kuroda A, Mizukami K, Hanada K, Okimoto T, Kodama M, Abe K, Yamaoka Y, Fujioka T
  • Journal Title: J Neurol Volume: 258(8) Pages: 1460-3
  • DOI: DOI:10.1007/s00415-011-5957-5
  • Peer Reviewed
• [Presentation] Association between serum Helicobacter pylori CagA antibody titer, pepsinogen in Japan (2012)
  • Author(s): Seiji Shiota, Kazunari Murakami, Yoshio Yamaoka, Toshio Fujioka
  • Organizer: Digestive Disease Week 2012
  • Place of Presentation: サンディエゴ、米国
  • Year and Date: 2012-05-20
• [Presentation] Helicobacter pylori dupA is the predictor for the failure of primary eradication (2011)
  • Author(s): Seiji Shiota, Lam Tung Nguyen, Kazunari Murakami, Toshio Fujioka, Yoshio Yamaoka
  • Organizer: Digestive Disease Week 2011
  • Place of Presentation: シカゴ、米国
  • Year and Date: 2011-05-10
• [Book] Helicobacter pylori 感染率と感染経路Helicobacter Research (2013)
  • Author(s): 塩田星児, 山岡吉生
  • Total Pages: 94-99
  • Publisher: 先端医学社
• [Book] 米国消化器病週間 (Digestive Disease Week:DDW) 2012 -基礎から-Helicobacter Research (2012)
  • Author(s): 塩田星児
  • Total Pages: 441-444
  • Publisher: 先端医学社
• [Book] プライマリーケアにおける上腹部症状と内視鏡所見消化器内科 (2012)
  • Author(s): 塩田星児, 村上和成
  • Total Pages: 7-11
  • Publisher: 科学評論社
• [Book] 傷害機序に関する新知見をみるHelicobacter Research (2011)
  • Author(s): 塩田星児, 山岡吉生
  • Total Pages: 319-324
  • Publisher: 先端医学社
• [Book] Helicobacter pylori infection in JapanHelicobacter Research (2011)
  • Author(s): Seiji Shiota, Kazunari Murakami, Yoshio Yamaoka, Toshio Fujioka
  • Total Pages: 201-207
  • Publisher: 先端医学社