2012 Fiscal Year Final Research Report

To explore the biomarkers which determine the quality of high density lipopotein

Research Project

Project/Area Number	23790853
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Circulatory organs internal medicine
Research Institution	Kobe University
Principal Investigator	YASUDA Tomoyuki 神戸大学, 医学部附属病院, 特定助教 (20457047)
Project Period (FY)	2011 – 2012
Keywords	分子血管病態学
Research Abstract	I n the present study, we used rabbit’s and human’s samples for evaluating HDL quality. In basic study, we used the myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHL-MI) rabbits which develop atherosclerotic lesion by month and control Japanese white (JW) rabbits which are healthy control. We evaluated the quantitative or qualitative alteration of HDL in the same individual rabbit. Paraoxonase activity assay, as an antioxidant property of HDL, and cholesterol efflux capacity assay were performed for evaluating HDL quality. There were no differences in HDL quality between 2 rabbit models. HDL qualitative evaluation was performed using the dyslipidemia patients’ serum before and after statin treatment. Statin increased HDL-C levels, and the cholesterol efflux capacity and antioxidant potential of increased HDL were maintained. As mentioned above, both HDL quantities and qualities analysis would be important for evaluating the risk of atherosclerosis.