2012 Fiscal Year Final Research Report

The roles of transcriptional regulation of angiogenesis and tissue repair in cell transplantation

Research Project

Project/Area Number	23790870
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Circulatory organs internal medicine
Research Institution	Nara Medical University
Principal Investigator	HAYASHI Hisaki 奈良県立医科大学, 医学部, 助教 (30533715)
Project Period (FY)	2011 – 2012
Keywords	血管新生 / 転写調節因子 / 虚血 / シグナル伝達 / 細胞移植
Research Abstract	In a mouse model of pathological angiogenesis by hindlimb ischemia (HLI) with femoral artery occlusion, the mRNA expression of Hrt1was markedly induced in ischemic muscle. Hrt1 KO mice do not show overt cardiovascular phenotypes in adulthood, but blood flow recovery in ischemic hindlimb after HLI was significantly blunted compared to WT. Revascularization of ischemic hindlimb was also attenuated in Hrt1 KO mice, as evaluated by capillary density analysis. Accordingly, the severity of ischemic injury such as tissue necrosis and loss is much higher in Hrt1 KO mice than WT. These results suggest that Hrt1 plays important roles in regulating ischemia-induced angiogenesis.