2012 Fiscal Year Final Research Report
Comprehensive analysis of cardiomyopathy-related signaling networks based on quantitative phosphoproteomics
| Project/Area Number |
23790889
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 心筋症 / リン酸化プロテオミクス / 定量プロテオミクス |
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| Research Abstract |
In this study, a novel method for reproducible, unbiased, and quantitative extraction of tissue proteins was successfully developed. Using this method combined with an optimized protocol for phosphopeptide enrichment and LC-MS/MS analysis, more than 6,000 phosphopeptides were identified and quantified from two kinds of mouse models for cardiomyopathy and a control one, and 20% of the identified peptides showed more than two-fold changes in MS signals. From these data, I am attempting to identify specific signal networks closely related to the onset and development of cardiomyopathy.
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[Journal Article]2013
Author(s)
Ryota Yamana, Mio Iwasaki, Masaki Wakabayashi, Masato Nakagawa, Shinya Yamanaka, Yasushi Ishihama
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Journal Title
Journal of Proteome Research
Volume: 12
Pages: 214-221
DOI
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