Differentiation into airway epithelial cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23790899
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: AKIRA Saito 東京大学, 保健・健康推進本部, 助教 (90591412)
• Project Period (FY): 2011 – 2013
• Keywords: 遺伝子導入 / 分化誘導

Research Abstract

In order to promote differentiation of human iPS (induced pluripotent stem) cells into lung epithelial cells, we tested the effects of various culture conditions, including inhibitors, growth factors and culture dish coating. We generated episomal vectors for TTF-1 (thyroid transcription factor-1), TAZ (transcriptional co-activator with PDZ-binding motif) and FOXA2 (forkhead box A2), and these genes were transduced into iPS cells or human skin fibroblasts by electroporation. Upregulation of markers for lung epithelial cells were examined by qRT-PCR (quantitative RT-PCR), and cell morphology of differentiated cells were investigated by electron microscopy.

Research Products

• [Journal Article] Histamine induces human lung fibroblast-mediated collagen gel contraction via histamine H1 receptor (2014)
  • Author(s): Horie M, Saito A, Yamauchi Y, Mikami Y, Sakamoto M, Jo T, Nakajima J, Takizawa H, Nagase T, Kohyama T
  • Journal Title: Exp Lung Res Volume: 40(5) Pages: 222-36
  • DOI: 10.3109/01902148.2014.900155
  • Peer Reviewed
• [Journal Article] An integrated expression profiling reveals target genes of TGF-βand TNF-αpossibly mediated by microRNAs in lung cancer cells (2013)
  • Author(s): Saito A, Suzuki HI, Horie M, Ohshima M, Morishita Y, Abiko Y, Nagase T
  • Journal Title: PLoS One Volume: 8(2) Pages: e56587
  • DOI: 10.1371/annotation/874e0b87-0383-45ec-a250-3a4cd087dc86
  • Peer Reviewed
• [Journal Article] EMT and EndMT : regulated in similar ways? (2013)
  • Author(s): Saito A
  • Journal Title: J Biochem Volume: 153(6) Pages: 493-5
  • DOI: 10.1093/jb/mvt032
  • Peer Reviewed
• [Journal Article] CD99 is a novel prognostic stromal marker in non-small cell lung cancer (2012)
  • Author(s): Edlund K, Lindskog C, Saito A, Berglund A, Pontén F, Göransson-Kultima H, Isaksson A, Jirström K, Planck M, Johansson L, Lambe M, Holmberg L, Nyberg F, Ekman S, Bergqvist M, Landelius P, Lamberg K, Botling J, Ostman A, Micke P
  • Journal Title: Int J Cancer Volume: 131(10) Pages: 2264-73
  • DOI: 10.1002/ijc.27518
  • Peer Reviewed
• [Journal Article] Characterization of human lung cancer-associated fibroblasts in three-dimensional in vitro co-culture model (2012)
  • Author(s): Horie M, Saito A, Mikami Y, Ohshima M, Morishita Y, Nakajima J, Kohyama T, Nagase T
  • Journal Title: Biochem Biophys Res Commun Volume: 423(1) Pages: 158-63
  • DOI: 10.1016/j.bbrc.2012.05.104
  • Peer Reviewed
• [Presentation] An integrated expression profiling reveals target genes of TGF-beta and TNF-alpha possibly mediated by microRNAs in lung cancer cells (2013)
  • Author(s): Akira Saito
  • Organizer: American Thoracic Society
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2013-05-20
• [Presentation] An integrated expression profiling reveals target genes of TGF-beta and TNF-alpha possibly mediated by microRNAs in lung cancer cells (2012)
  • Author(s): Akira Saito
  • Organizer: International Cancer Microenvironment Society
  • Place of Presentation: Suzhou, China
  • Year and Date: 2012-11-15