2012 Fiscal Year Final Research Report
Effect of TGF-β on WT1 expression in podocyte.
| Project/Area Number |
23790930
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Kidney internal medicine
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
SAKAIRI Toru 群馬大学, 医学部, 助教 (20455976)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 糖尿病性腎症 / 巣状糸球体硬化症 / 糸球体上皮細胞 / TGF-beta1 / WT1 |
|---|
| Research Abstract |
TGF-beta1 is involved in diabetes nephropathy and focal segmental glomerulonephritis. Transcriptional factor Wilms tumor suppressor gene (WT1) is essential for normal function of podocytes. We found that TGF-beta1 reduces WT1 expression in both cultured humanpodocytes and mouse podocytes. Using the cultured podocytes, we identified following mechanisms by which TGF-beta1 suppress WT1 expression. First, Smad4 mediates a decrease in WT1 expression by TGF-beta1. Second, TGF-beta1 caused DNA methylation in promoter region of the podocytes. Our results indicate that diverse pathways contribute to the TGF-beta1-induced WT1 reduction that may result in podocyte injury.
|
Research Products
(5 results)
