Analysis of adipocyte-specific transcriptional gene regulation by using next generation sequencer

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791020
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Metabolomics
• Research Institution: The University of Tokyo
• Principal Investigator: WAKI Hironori 東京大学, 医学部附属病院, 特任准教授 (00466765)
• Project Period (FY): 2011 – 2012
• Keywords: 分化 / 遺伝子 / ゲノム / 糖尿病 / 脂肪細胞

Research Abstract

We generated genome-wide maps of open chromatin, histone modifications and PPARγ binding sites in 3T3-L1 adipocytes using FAIRE-seq and ChIP-seq. The data suggest that regulatory elements located distal to target genes play a critical role in adipocyte-specific gene regulation. We also demonstrated that a combination of motif analysis and FAIRE-seq provides unbiased identification of regulators of adipocyte-specific gene expression and reported identification of the NFI transcription family as a novel regulator of adipocyte differentiation.

Research Products

• [Journal Article] The Epigenome and Its Role in Diabetes (2012)
  • Author(s): Waki H, Yamauchi T, Kadowaki T
  • Journal Title: Curr Diab Rep Volume: 7
  • DOI: DOI:10.1007/s11892-012-0328-x
• [Journal Article] Global Mapping of Cell Type-Specific Open Chromatin by FAIRE-seq Reveals the Regulatory Role of the NFI Family in Adipocyte Differentiation (2011)
  • Author(s): Hironori Waki, Masahiro Nakamura, Toshimasa Yamauchi, Ken-ichi Wakabayashi, Jing Yu, Lisa Hirose-Yotsuya, Kazumi Take, Wei Sun, Masato Iwabu, Miki Okada-Iwabu, Takanori Fujita, Tomohisa Aoyama, Shuichi Tsutsumi, Kohjiro Ueki, Tatsuhiko Kodama, Juro Sakai, Hiroyuki Aburatani, Takashi Kadowaki
  • Journal Title: PLoS Genet Volume: 7(10) Pages: e1002311
  • Peer Reviewed
• [Presentation] Global Mapping of Cell-Type-Specific Open Chromatin by FAIRE-seq Reveals the Regulatory Role of the NFI Family in Adipocyte Differentiation (2012)
  • Author(s): Hironori Waki, Masahiro Nakamura, Toshimasa Yamauchi, , Ken-ichi Wakabayashi, Jing Yu, Lisa Hirose Yotsuya, Kazumi Take, Wei Sun, Masato Iwabu, Miki Okada-Iwabu, , Takanori Fujita, Tomohisa Aoyama, Shuichi Tsutsumi, Kohjiro Ueki, Tatsuhiko Kodama, Juro Sakai, Hiroyuki Aburatani, and Takashi Kadowaki
  • Organizer: Keystone Symposia Genetic and Molecular Basis of Obesity and Body Weight Regulation (J7) Epigenetics
  • Place of Presentation: Santa Fe
  • Year and Date: 20120129-0203
• [Presentation] ワークショップ:代謝と生活習慣病をつなぐエピ ジェネティクス制御機構 Epigenetic mechanism linking metabolism and life-style disease 脂肪細胞分化におけるクロマチン構造変化とエビゲノム制御の役割 (2012)
  • Author(s): 脇裕典
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 【Adiposcienceの新展開:肥満症の基礎研究】脂肪細胞分化におけるクロマチン構造変化とエビゲノム制御の役割 (2012)
  • Author(s): 脇裕典、中村正裕、山内敏正、若林賢一、于静、四谷理沙、武和巳、孫威、岩部真人、岡田美紀、藤田隆教、青山倫久、堤修一、植木浩二郎、児玉龍彦、酒井寿郎、油谷浩幸、門脇孝
  • Organizer: 第33回日本肥満学会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-12
• [Presentation] 脂肪細胞分化におけるクロマチン構造変化とエピゲノム制御の役割 (2012)
  • Author(s): 脇裕典
  • Organizer: 第17回アディポサイエンス研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-08-25
• [Book] 糖尿病学2012 (2012)
  • Author(s): 脇裕典
  • Total Pages: 35-43
• [Book] 肥満研究 (2012)
  • Author(s): 脇裕典
  • Total Pages: 61-65
• [Book] 医学のあゆみ (2012)
  • Author(s): 脇裕典、山内敏正、門脇孝
  • Total Pages: 918-923
• [Remarks]
  • URL: http://researchmap.jp/hironori.waki/?lang=japanese