2012 Fiscal Year Final Research Report
Elucidation of Pathogenesis and Development of a Cell Therapy in Lipodystrophy Using Human iPS Cells
| Project/Area Number |
23791031
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脂肪萎縮症 / ヒトiPS細胞 / 疾患特異的iPS細胞 / 細胞移植 |
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| Research Abstract |
Patient-specific iPS cells were generated from human dermal fibroblasts of 8 lipodystrophic patients by retroviruses containing human Oct3/4, Sox2, Klf4 and c-Myc. These patients included 5 patients with congenital generalized lipodystrophy, 1 patient with familial partial lipodystrophy and 2 patients with acquired lipodystrophy. Adipogenic differentiation properties of several patient-specific iPS cell lines were investigated. After in vitro adipogenic differentiation, differentiated human iPS cells were transplanted into the subcutaneous area of nude mice. The study demonstrated that adipocytes derived from human iPS cells can survive and maintain the differentiated properties in vivo for at least 4 weeks after transplantation.
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[Journal Article] Induced pluripotent stem cells generated from diabetic patients with mitochondrial DNA A3243G mutation.2012
Author(s)
J. Fujikura, K. Nakao, M. Sone, M. Noguchi, E. Mori, M. Naito, D. Taura, M. Harada-Shiba, I. Kishimoto, A. Watanabe, I. Asaka, K. Hosoda, K. Nakao.
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Journal Title
Diabetologia
Volume: 55
Pages: 1689-98
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