2012 Fiscal Year Final Research Report
Comprehensive analysis of molecular targets of autophagy in β cell
| Project/Area Number |
23791043
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | オートファジー / 膵 β 細胞 / プロテオミクス |
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| Research Abstract |
To comprehensively find molecular targets of autophagy in β cell, we compared protein expressi on profile in islets from pancreatic β-cell specific autophagy deficient mice (Atg7^-cell) and control mice (Atg7^). We identified ERp57 (PDIA3) significantly increased in Atg7^-cellisletsTo investigate the role of ERp57 in autophagy deficient β cells, we generated tetracycline-inducible Atg7 knock-down (Atg7KD) rat insulinoma INS-1 cells. Then, we additively reduced ERp57 in using siRNA in both INS-1 cells to investigate cell viabilities by sub-G1 analysis. The suppression of ERp57 caused to increase sub-G1 population in Atg7KD INS-1 cells. These data suggested that ERp57 might be induced by autophagy deficiency in β cells and ERp57 had protective effects on autophagy deficient β cells against apoptotic cell death.
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