Exploration of the mechanisms controlling ghrelin production and secretion by using a novel ghrelin-producing cell line.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: IWAKURA Hiroshi 京都大学, 医学研究科, 准教授 (20378615)
• Project Period (FY): 2011 – 2012
• Keywords: グレリン / 細胞株 / 分泌調節

Research Abstract

In this study, we examined the effects of nutrients on ghrelin secretion by original ghrelin secreting cell line, MGN3-1. Glucose showed no apparent effect on ghrelin secretion. Octanoic acid increased active-ghrelin secretion, while long-chain fatty acids suppressed it. Certain amino acids stimulated ghrelin secretion. These results indicate that nutrients have certain roles in regulation of ghrelin secretion.

Research Products

• [Journal Article] Adrenal tumor volume in a genetically engineered mouse model of neuroblastoma determined by magnetic resonance imaging. (2012)
  • Author(s): Kawano K, Hattori Y, Iwakura H, Akamizu T, Maitani Y
  • Journal Title: Exp Ther Med. Volume: 4(1) Pages: 61-64
  • DOI: doi:10.3892/etm.2012.564
  • Peer Reviewed
• [Journal Article] Transgenic mice overexpressing ghrelin or ghrelin analog. (2012)
  • Author(s): Ariyasu H, Yamada G, Iwakura H, Akamizu T, Kangawa K, Nakao K.
  • Journal Title: Methods Enzymol. Volume: 514 Pages: 371-7
  • DOI: doi:1016/B978-0-12-381272-8.00023-4.
• [Journal Article] Sensing of fatty acids for octanoylation of ghrelin involves a gustatory G-protein. (2012)
  • Author(s): Janssen S, Laermans J, Iwakura H, Tack J, Depoortere I.
  • Journal Title: PLoS One Volume: 7(6) Pages: e40168
  • DOI: doi:10.1371/journal.pone.0040168.
  • Peer Reviewed
• [Journal Article] Transgenic overexpression of intra-islet ghrelin does not affect insulin secretion or glucose metabolism in vivo. (2012)
  • Author(s): Bando M, Iwakura H, Ariyasu H, Hosoda H, Yamada G, Hosoda K, Adachi S, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Am J Physiol Endocrinol Metab. Volume: 302(4) Pages: E403-8
  • DOI: doi:10.1152/ajpendo.00341.2011
  • Peer Reviewed
• [Journal Article] nalysis of plasma ghrelin in patients with medium-chain acyl-CoA dehydrogenase deficiency and glutaric aciduria type II. (2012)
  • Author(s): Akamizu T, Sakura N, Shigematsu Y, Tajima G, Ohtake A, Hosoda H, Iwakura H, Ariyasu H, Kangawa K.
  • Journal Title: Eur J Endocrinol. Volume: 166(2) Pages: 235-40
  • DOI: doi:10.1530/EJE-11-0785
  • Peer Reviewed
• [Journal Article] Oxytocin and dopamine stimulate ghrelin secretion by the ghrelin-producing cell line, MGN3-1 in vitro. (2011)
  • Author(s): Iwakura H, Ariyasu H, Hosoda H, Yamada G, Hosoda K, Nakao K, Kangawa K, Akamizu T.
  • Journal Title: Endocrinology. Volume: 152(7) Pages: 2619-25
  • DOI: doi:10.1210/en.2010-1455
  • URL: http://repository.kulib.kyoto-u.ac.jp/dspace/handle/2433/158372
  • Peer Reviewed
• [Journal Article] 加齢と肥満 (2011)
  • Author(s): 岩倉 浩
  • Journal Title: 最新医学 Volume: 66 Pages: 828-832
  • Peer Reviewed
• [Presentation] グレリン産生細胞株樹立によるグレリン生合成、分泌機構の解明、 (2013)
  • Author(s): 岩倉 浩
  • Organizer: 第86回日本内分泌学会学術総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2013-04-26
• [Presentation] アミノ酸による in vitro でのグレリン分泌調節機構の解明 (2013)
  • Author(s): 小山博之、岩倉 浩、細田洋司、坂東美佳、細田公則、寒川賢治、中尾一和、
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2013-04-25
• [Presentation] 膵島内グレリン過剰発現がストレプトゾトシン誘導β細胞障害に与える影響の検討、 (2012)
  • Author(s): 坂東美佳, 岩倉 浩, 有安宏之, 細田 公則, 足立壯一, 中尾一和, 寒川賢治, 赤水尚史、
  • Organizer: 第33回日本肥満学会、
  • Place of Presentation: ホテルグランビア京都
  • Year and Date: 2012-10-11
• [Presentation] ストレプトゾトシンによるβ細胞障害に対する膵島内グレリン過剰発現の影響の検討、 (2012)
  • Author(s): 坂東美佳, 岩倉 浩, 有安宏之, 細田 洋司, 山田豪, 細田公則, 足立壯一, 中尾 一和, 寒川 賢治, 赤水 尚史
  • Organizer: 第55回日本糖尿病学会年次学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2012-05-19
• [Presentation] 膵島内グレリン、GOAT 過剰発現トランスジェニックマウスの解析、 (2011)
  • Author(s): 坂東美佳, 岩倉 浩, 有安宏之, 細田公則, 足立壯一, 中尾一和, 寒川賢治, 赤水尚史、
  • Organizer: 第32回日本肥満学会、
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2011-09-23
• [Presentation] グレリン分泌細胞の樹立と in vitro でのグレリン分泌調節の検討、 (2011)
  • Author(s): 岩倉 浩, 有安宏之, 細田洋司, 細田公則, 中尾一和, 寒川賢治, 赤水尚史、
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌プリンスホテル(北海道)
  • Year and Date: 2011-05-19
• [Book] 最新内分泌学 (2013)
  • Author(s): 岩倉 浩、他
  • Total Pages: 528-530, 792-794
  • Publisher: 診断と治療社
• [Book] レプチンのトランスレーショナルサイエンス-メタボリックシンドロームの治療戦略、 (2012)
  • Author(s): 岩倉 浩、他
  • Total Pages: 43-45, 49-52
  • Publisher: 診断と治療社
• [Book] Springer (2011)
  • Author(s): Hiroshi Iwakura, 他
  • Total Pages: 3
  • Publisher: Neuroblastoma mouse model, Neuroblastoma (Pediatric Cancer)
• [Book] 症候群ハンドブック
  • Author(s): 岩倉 浩、他
  • Total Pages: 375
  • Publisher: 中山書店