2012 Fiscal Year Final Research Report
Identification and functional analysis of novel bioactive peptides that regulate energy metabolism and food intake
| Project/Area Number |
23791066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
YOSHIDA Morikatsu 独立行政法人国立循環器病研究センター, 研究所, 研究員 (70393212)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生理活性ペプチド / オーファン受容体 / 摂食・エネルギー代謝調節 |
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| Research Abstract |
We performed a search for newly bioactive peptides acting on orphan receptors expressed in the hypothalamus which controls the energy metabolism and feeding behavior. For screening candidate ligands of these receptors, we developed novel activity detection system which is determined by microscopic morphological changes of cells (CellKey assay). Candidate ligands in tissue extracts were screened against cell lines expressing target orphan receptors by using this system. In cells expressing a certain orphan receptor, we detected the significant increase of impedance, which was the target receptor specific. We are now proceeding to purify the active peptide from this extract.
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