2012 Fiscal Year Final Research Report
Analysis of function of microRNAs in Dlk1-Dio3 region in hematopoietic stem cells
| Project/Area Number |
23791075
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMAMOTO Ryo 東京大学, 医科学研究所, 特任研究員 (00581191)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 造血幹細胞 / 自己複製能 / 多分化能 / microRNA |
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| Research Abstract |
Hematopoietic stem cells (HSCs, CD34-KSL) have self-renewal activity and multipotency. CD150+ cells in CD34-KSL fraction have long-term and myeloid-biased repopulating activity. We have identified microRNAs specific to CD150+CD34-KSL fraction and transplanted microRNA-transduced CD34-KSLs into lethally-irradiated mice. However, we could not detect microRNAs that expanded self-renewal activity. This is, in part, due to HSC heterogeneity because candidate microRNAs have been reported to be expressed in megakaryocytes. To assess this, we performed single-cell transplantation of bone marrows from mice in which all mature blood cells express fluorescent protein. We detected myeloid-restricted repopulating cells in addition to full-lineage HSC in HSC fraction, suggesting that some of candidate microRNAs have a role in lineage commitment rather than self-renewal.
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