Identification of new autoantibodies in anti-cyclic citrullinated peptide (CCP) antibodies negative rheumatoid arthritis

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791112
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kyoto University
• Principal Investigator: YUKAWA Naoichiro 京都大学, 大学院・医学研究科臨床免疫学, 助教 (90422972)
• Project Period (FY): 2011 – 2012
• Keywords: リウマチ学 / 自己抗体

Research Abstract

The new autoantibodies were screened by the AlphaScreen methodusing sera from eight antiCCP antibody-negative rheumatoid factor-negative rheumatoid arthritis (RA) patients. In two candidate proteins which were detected in all sera by AlphaScreen method, the autoantibodies were determined by Western blot and ELISA. Although autoantibodies were detected by Western blot, the detection rate was low by ELISA. These antibodies may become the new autoantibody in the autoantibody-negative RA. Next, we are going to examine the clinical utility of these autoantibodies.

Research Products

• [Journal Article] Neutrophils are essential as a source of IL-17 in the effector phase of arthritis. (2013)
  • Author(s): Katayama M, Ohmura K, Yukawa N, et al. (他 7 名 , 3 番 目)
  • Journal Title: PLoS One Volume: 8 Pages: e62231
  • DOI: doi:10.1371/journal.pone. 0062231
  • Peer Reviewed
• [Journal Article] Three groups in the 28 joints for rheumatoid arthritis synovitis--analysis using more than 17,000 assessments in the KURAMA database. (2013)
  • Author(s): Terao C, Hashimoto M, Yukawa N, et al. (他 15 名)
  • Journal Title: PLoS One. Volume: 8 Pages: e59341
  • Peer Reviewed
• [Journal Article] Living-donor lobar lung transplantation for rapidly progressive interstitial pneumonia associated with clinically amyopathic dermatomyositis: report of a case. (2013)
  • Author(s): Shoji T, Bando T, Yukawa N, et al. (他5 名)
  • Journal Title: Gen Thorac Cardiovasc Surg. Volume: 61 Pages: 32-4
  • DOI: doi:10.1007/s11748- 012-0106-3.
  • Peer Reviewed
• [Journal Article] Serum BAFF and APRIL levels in patients with IgG4-related disease and their clinical significance. (2012)
  • Author(s): Kiyama K, Kawabata D, Yukawa N, et al.
  • Journal Title: Arthritis Res Ther. Volume: 14 Pages: R86
  • DOI: doi:10.1186/ar3810
  • Peer Reviewed
• [Journal Article] Follistatin-related protein / follistatin -like 1 evokes an innate immune response via CD14 and tolllike receptor 4. (2012)
  • Author(s): Murakami K, Tanaka M, Yukawa N, et al. (他 11 名)
  • Journal Title: FEBS Lett Volume: 586 Pages: 319-24
  • Peer Reviewed
• [Journal Article] Serum IgG levels demonstrate seasonal change in connective tissue diseases: a large-scale, 4-year analysis in Japanese. (2012)
  • Author(s): Terao C, Ohmura K, Yukawa N, et al. (他5 名)
  • Journal Title: Mod Rheumatol Volume: 22 Pages: 426-30
  • DOI: doi:10.1007/s10165-011- 0535-3.
  • Peer Reviewed
• [Journal Article] Correlation of antinuclear antibody and anti-double-stranded DNA antibody with clinical response to infliximab in patients with rheumatoid arthritis: a retrospective clinical study. (2011)
  • Author(s): Yukawa N, Fujii T, Kondo-Ishikawa S, et al.
  • Journal Title: Arthritis Res Ther. Volume: 13 Pages: R213
  • DOI: doi:10.1186/ ar3546.
  • Peer Reviewed
• [Journal Article] Overexpression of a minimal domain of calpastatin suppresses IL-6 production and Th17 development via reduced NF-κB and increased STAT5 signals. (2011)
  • Author(s): Iguchi-Hashimoto M, Usui T, Yukawa N, et al.(他 11 名)
  • Journal Title: PLoS One Volume: 6 Pages: e27020
  • DOI: doi:10.1371/ journal.pone. 0027020.
  • Peer Reviewed
• [Journal Article] Interferon-gamma release assay for diagnosing Mycobacterium tuberculosis infections in patients with systemic lupus erythematosus. (2011)
  • Author(s): Takeda N, Nojima T, Yukawa N, et al. (他 8 名)
  • Journal Title: Lupus. Volume: 20 Pages: 792-800.
  • DOI: doi:10.1177/ 0961203310397966.
  • Peer Reviewed
• [Presentation] 関節リウマチにおける抗 MBP 抗体のエピトープとその意義. (2012)
  • Author(s): 大村 浩一郎, 山本 奈つき, 寺尾 知可史, 中嶋 蘭, 井村 嘉孝, 吉藤 元, 湯川 尚一郎, 橋本 求, 藤井 隆夫, 松田 文彦, 三森 経世
  • Organizer: 日本臨床免疫学会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 20120927-29
• [Presentation] ホリスタチン関連タンパク質は CD14 と Toll 様受容体 4 を介して自然免疫反応を惹起する. (2012)
  • Author(s): 村上 孝作, 田中 真生, 臼井 崇, 川端 大介, 塩見 葵, 井口 美季子, 清水 正和, 吉藤 元, 湯川 尚一郎, 大村 浩一郎, 藤井 隆夫, 梅原 久範, 三森 経世.
  • Organizer: 日本臨床免疫学会
  • Place of Presentation: 東京都新宿区
  • Year and Date: 20120927-29
• [Presentation] NPSLE 患者髄液中における抗 U1RNP 抗体と液性因子との相関. (2012)
  • Author(s): 横山 倫子, 藤井 隆夫, 山川 範之, 中嶋 蘭, 村上 孝作, 湯川 尚一郎, 橋本 求, 吉藤 元, 川端 大介, 大村 浩一郎, 三森 経世
  • Organizer: 日本リウマチ学会総会・学術集会・国際リウマチシンポジウム
  • Place of Presentation: 東京都品川区
  • Year and Date: 20120426-28
• [Presentation] K/BxN 関節炎モデルマウスにおいて,IL-17は関節炎に影響せず軟骨破壊に作用する. (2012)
  • Author(s): 片山 昌紀, 大村 浩一郎, 寺尾 知可史, 山本 奈つき, 橋本 求, 湯川 尚一郎, 吉藤 元, 川端 大介, 藤井 隆夫, 三森 経世.
  • Organizer: 日本リウマチ学会総会・学術集会・国際リウマチシンポジウム
  • Place of Presentation: 東京都品川区
  • Year and Date: 20120426-28
• [Presentation] 関節リウマチにおけるリウマトイド因子・抗 CCP 抗体の抗体価と臨床経過との相関. (2012)
  • Author(s): 村上 孝作, 藤井 隆夫, 寺尾 知可史, 大村 浩一郎, 川端 大介, 吉藤 元, 湯川 尚一郎, 橋本 求, 三森 経世.
  • Organizer: 日本リウマチ学会総会・学術集会・国際リウマチシンポジウム
  • Place of Presentation: 東京都品川区
  • Year and Date: 20120426-28
• [Presentation] 自己抗体 抗アミノアシル tRNA 合成酵素抗体を検出するための新規ELISA の開発. (2011)
  • Author(s): 中嶋蘭, 井村嘉孝, 瀬戸美苗, 村上昭弘, 小林志緒, 細野祐司, 湯川尚一郎, 吉藤元, 川端大介, 大村浩一郎, 臼井崇, 藤井隆夫, 三森経世
  • Organizer: 第39回日本臨床免疫学会総会(シンポジウム)
  • Place of Presentation: 東京都新宿区
  • Year and Date: 2011-09-16
• [Book] アダリムマブ/ヒュミラ.インフォームドコンセントのための図説シリーズ 関節リウマチ -生物学的製剤の正しい使い方とは? (2011)
  • Author(s): 湯川尚一郎,三森経世(宮坂信之編)
  • Total Pages: 84
  • Publisher: 医薬ジャーナル社