2013 Fiscal Year Final Research Report
The development of immune-modulate molecule using the binding specificity of Toll-Interleukin-1 receptor domain
| Project/Area Number |
23791161
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Gifu University |
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Principal Investigator |
OHNISHI Hidenori 岐阜大学, 医学(系)研究科(研究院), 助教 (60381620)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 自然免疫 |
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| Research Abstract |
In this study, we aimed the development of the molecule, which modulates the Toll-Interleukin-1 receptor (TIR) signaling using the binding specificity of TIR domain of MyD88. The dominant negative form (DN) of MyD88 fused with protein transduction domain (PTD) was purified with E. Coli protein expression system. In order to stabilize this protein, two cysteine amino acids of MyD88, C203 and C280, were mutated to serine (CS). PTD-MyD88-DN-CS protein caused the ligand-independent increase of transcriptional activity of NF-kappaB.
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[Journal Article] Autosomal dominant anhidrotic ectodermal dysplasia with immunodeficiency caused by a novel NFKBIA mutation, p.Ser36Tyr, presents with mild ectodermal dysplasia and non-infectious systemic inflammation2013
Author(s)
Yoshioka T, Nishikomori R, Hara J, Okada K, Hashii Y, Okafuji I, Nodomi S, Kawai T, Izawa K, Ohnishi H, Yasumi T, Nakahata T, Heike T
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Journal Title
J Clin Immunol
Volume: 33(7)
Pages: 1165-74
Peer Reviewed
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[Journal Article] Autosomal-dominant chronic mucocutaneous candidiasis with STAT1-mutation can be complicated with chronic active hepatitis and hypothyroidism2012
Author(s)
Hori T, Ohnishi H, Teramoto T, Tsubouchi K, Naiki T, Hirose Y, Ohara O, Seishima M, Kaneko H, Fukao T, Kondo N
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Journal Title
J Clin Immunol
Volume: 32(6)
Pages: 1213-20
Peer Reviewed
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[Journal Article] Molecular analysis of the binding mode of Toll/interleukin-1 receptor (TIR) domain proteins during TLR2 signaling2012
Author(s)
Nada M, Ohnishi H, Tochio H, Kato Z, Kimura T, Kubota K, Yamamoto T, Kamatari YO, Tsutsumi N, Shirakawa M, Kondo N
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Journal Title
Mol Immunol
Volume: 52(3-4)
Pages: 108-16
Peer Reviewed
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[Journal Article] TRAM is involved in IL-18 signaling and functions as a sorting adaptor for MyD882012
Author(s)
Ohnishi H, Tochio H, Kato Z, Kawamoto N, Kimura T, Kubota K, Yamamoto T, Funasaka T, Nakano H, Wong RW, Shirakawa M, Kondo N
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Journal Title
PLoS One
Volume: 7(6)
Pages: e38423
Peer Reviewed
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[Journal Article] Structural property of soybean protein P34 and specific IgE response to recombinant P34 in patients with soybean allergy2012
Author(s)
Morita H, Kaneko H, Ohnishi H, Kato Z, Kubota K, Yamamoto T, Matsui E, Teramoto T, Fukao T, Kasahara K, Kondo N
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Journal Title
Int J Mol Med
Volume: 29(2)
Pages: 153-8
Peer Reviewed
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