2012 Fiscal Year Final Research Report
Clarification of the mucosal immune system and development of mucosal immunotherapy in pediatric autoimmune diseases
| Project/Area Number |
23791185
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 小児免疫 / アレルギー / 膠原病学 |
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| Research Abstract |
Transforming growth factor-beta (TGF-β) plays a pivotal role in immunoregulation, yet the role of its intracellular signaling molecules, known as the Smads, had not been established. Previously, we demonstrated that both Smad2 and Smad3 redundantly play essential roles in the immunosuppressive function of TGF-β, including the induction of Foxp3, the master regulator of regulatory T cells. We made a reporter assay system with the luciferase gene under the control of the FOXP3 promoter and enhancer containing Smad-binding element. We confirmed that this reporter assay system worked in Jurkat cells, human T cell line. Furthermore, we demonstrated that not only SMAD3 but also SMAD2 can bind to the enhancer region on FOXP3 gene by using the ChIP assay.
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