2013 Fiscal Year Final Research Report

Identification of the causative antigens of idiopathic steroid-sensitive nephrotic syndrome in childhood by proteome analysis

Research Project

Project/Area Number	23791191
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Pediatrics
Research Institution	Jichi Medical University
Principal Investigator	JUN Odaka 自治医科大学, 医学部, 講師 (70382885)
Project Period (FY)	2011 – 2012
Keywords	小児特発性ネフローゼ症候群 / プロテオミクス / 蛋白透過性因子
Research Abstract	It is suggested that some humoral factors are the causative agents of idiopathic nephrotic steroid-sensitive nephrotic syndrome (ISSNS) in childhood. This study aimed to identify this humoral factors.Since a humoral factor component mainly of a protein, in this study, we used a proteomic analysis called surface-enhanced laser desorption/ionization time of flight mass spctrometry to identify serum proteins which is highly expressed in ISSNS patients. Apolipoprotein C1 was detected at significant elevation during the acute phase compared with the remission phase and pediatric patients with secondry nephrotic syndrome. This is the first report that shows a relationship between an elevation of apolipoprotein C1 and the pathphysiology of ISSNS, and this findings present an interesting starting point for further investigation into the pathophysiology of ISSNS.