A study of anti-Dsc autoantibodies for pemphigus herpetiformis and pemphigus vegetans

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kurume University
• Principal Investigator: ISHII Norito 久留米大学, 医学部, 講師 (80330827)
• Project Period (FY): 2011 – 2012
• Keywords: 皮膚診断学 / 疱疹状天疱瘡 / 増殖性天疱瘡 / 腫瘍随伴性天疱瘡 / デスモコリン / ELISA法

Research Abstract

No consistent immunoassay for IgG autoantibodies to Dscs has been developed. The aim of this study was to develop reliable assays to detect anti-Dsc autoantibodies. We expressed soluble recombinant proteins of human Dsc1-Dsc3 in mammalian cells and examined sera of various types of pemphigus by novel enzyme-linked immunosorbent assays (ELISAs) using the recombinant proteins. We also performed ELISAs of Dsc baculoproteins, cDNA transfection method, and compared the results with those of mammalian ELISAs.
We have successfully established new sensitive and specific mammalian Dsc IgG enzyme-linked immunosorbent assays (ELISAs). Our data show that IgG anti-Dsc autoantibodies are frequently detected and potentially pathogenic in particular types of non-classical pemphigus.

Research Products

• [Journal Article] Concurrence of bullous pemphigoid and herpetiform pemphigus with IgG antibodies to desmogleins 1/3 and desmocollins 1-3 (2013)
  • Author(s): Ohata C, Koga H, Teye K, Ishii N, Hamada T, Dainichi T, Furumura M, Sato M, Sueki H, Hashimoto T.
  • Journal Title: Br J Dermatol Volume: 168(4) Pages: 879-881
  • DOI: 10.1111/bjd.12019
  • Peer Reviewed
• [Journal Article] IgA pemphigus with paraneoplastic pemphigus-like clinical features showing IgA antibodies to desmoglein 1/3 and desmocollin 3, and IgG and IgA antibodies to the basement membrane zone (2013)
  • Author(s): Ueda A, Ishii N, Temporin K, Yamazaki R, Murakami F, Fukuda S, Hamada T, Dainichi T, Kyoya M, Saito C, Matsunaga R, Kimura S, Kawakami T, Soma Y, Hashimoto T.
  • Journal Title: Clin Exp Dermatol Volume: 38(4) Pages: 370-373
  • DOI: 10.1111/ced.12050
  • Peer Reviewed
• [Journal Article] Unique herpetiform bullous dermatosis with IgG antibodies to desmocollins 1/3 and LAD-1 (2013)
  • Author(s): Ueda A, Ishii N, Teye K, Dainichi T, Ohyama B, Hamada T, Tsuruta D, Kamioka N, Mitsui K, Hosaka H, Soh I, Nakada T, Hashimoto T.
  • Journal Title: Br J Dermatol Volume: 169(3) Pages: 719-721
  • DOI: 10.1111/bjd.12398
  • Peer Reviewed
• [Journal Article] Two cases of pemphigus vegetans with IgG anti-desmocollin 3 antibodies (2013)
  • Author(s): Saruta H, Ishii N, Teye K, Ono F, Ohyama B, Koga H, Ohata C, Furumura M, Tsuruta D, Hashimoto T.
  • Journal Title: JAMA Dermatol Volume: 149 Pages: 1209-13
  • DOI: 10.1001/jamadermatol.2013.5244
  • Peer Reviewed