2013 Fiscal Year Final Research Report
Global and target-oriented proteomic analysis in the postmortem brains from patients with schizophrenia
| Project/Area Number |
23791344
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Psychiatric science
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
KUNII Yasuto 福島県立医科大学, 医学部, 助教 (00511651)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTSUKI Sumio 熊本大学, 薬学部, 教授 (60323036)
AKATSU Hiroyasu 医療法人さわらび会福祉村病院, 長寿医学研究所, 副所長 (00399734)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 統合失調症 / 死後脳研究 / プロテオミクス |
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| Research Abstract |
We investigated the comparative analyses of protein expressions in the dorsolateral prefrontal cortex from patients with schizophrenia and healthy control subjects using novel methodology of proteomics based on the mass spectrometry, which consisted in two methods, global and target-oriented analysis. Global proteomic analysis resulted in the identification of 8,768 proteins, 300 of which showed statistically significant differential expression among two groups. Target-oriented analysis determined the quantities of the expression of 108 proteins which are believed to be associated with the pathogenesis of schizophrenia, and as a result several proteins showed significant differences among two groups. These proteins include both of the previously reported ones and newly founded ones in this study. Additionally, we examined the distributions, and compared the expressive intensities of these proteins in DLPFC among two groups using immunofluorescence staining.
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[Journal Article] Elevated postmortem striatal t-DARPP expression in schizophrenia and associations with DRD2/ANKK1 polymorphism2014
Author(s)
Kunii Y, Miura I, Matsumoto J, Hino M, Wada A, Niwa SI, Nawa H, Sakai M, Someya T, Takahashi H, Kakita A, Yabe H.
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Journal Title
Prog Neuropsychopharmacol Biol Psychiatry
Volume: 5846(14)(Epub ahead of print)
Pages: 00075-X
DOI
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[Presentation] Expression levels of CHRNA7 and CHRFAM7A are altered in postmortem dorsolateral prefrontal cortex in major psychiatric disorder2013
Author(s)
Yasuto Kunii, Thomas M.Hyde, Tlanzhang Ye, Chao Li, Ran Tao, Whitney McFadden, Daniel R.Weinberger,Joel E.Kleinman,and Barbara K.Lipska
Organizer
Neuroscience
Place of Presentation
SanDiego,CA,USA
Year and Date
2013-11-11
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[Presentation] Effects of the Taq1A Polymorphism in the Dopamine D2 Receptor Gene on the DARPP-32 Expression of Postmortem Striatum2013
Author(s)
Yasuto Kunii, Itaru Miura, Junya Matsumoto, Mizuki Hino, Akira Wada, Hirooki Yabe, Akiyoshi Kakita, Hitoshi Takahashi, Miwako Sakai, Toshiyuki Someya, Hiroyuki Nawa, Shin-ichi Niwa.
Organizer
11th World Congress of Biological Psychiatry
Place of Presentation
kyoto
Year and Date
2013-06-26
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