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2012 Fiscal Year Final Research Report

Novel synthetic approach for a positron emitter labeled protein by cell free protein synthesis system

Research Project

  • PDF
Project/Area Number 23791387
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Radiation science
Research InstitutionTohoku University

Principal Investigator

FURUMOTO Shozo  東北大学, 大学院医学系研究科, 准教授 (00375198)

Project Period (FY) 2011 – 2012
Keywords放射性医薬品 / 造影剤
Research Abstract

In this study, we evaluated a novel synthetic approach for a positron emitter labeled protein by cell-free protein synthesis (CFPS) system. Synthesis of ^<11>C-GFP using ^<11>C-Met and CFPS afforded the product within a short time. On the other hand, when using ^<18>F-Pro, the ^<18>F-labeled GFP was produced too slowly to use it practically. We succeeded to prepare^<11>C-labeled scFv antibody to EGFR, which showed specific binding to EGFR-positive tumorcells in vitro and specific uptake by the EGFR-positive tumor in vivo. These results suggest the usefulness of the ^<11>C-labeld protein synthesis by CFPS.

  • Research Products

    (4 results)

All 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (1 results)

  • [Journal Article] Rapid biochemical synthesis of (11)C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis2012

    • Author(s)
      Matsuda T, Furumoto S, Higuchi K, Yokoyama J, Zhang MR, Yanai K, Iwata R, Kigawa T.
    • Journal Title

      Bioorg Med Chem

      Volume: 20(22) Pages: 6579-6582

    • Peer Reviewed
  • [Journal Article] Reactivity of electrochemically concentrated anhydrous [^<18>F]fluoride for microfluidic radiosynthesis of ^<18>F-labeled compounds2012

    • Author(s)
      Wong R, Iwata R, Saiki H, Furumoto S, Ishikawa Y, Ozeki E.
    • Journal Title

      Appl Radiat Isot

      Volume: 70(1) Pages: 193-199

    • Peer Reviewed
  • [Journal Article] Synthesis of [^<11>C]interleukin 8 using a cell-free translation system and L-[^<11>C]methionine2012

    • Author(s)
      Harada R, Furumoto S, Yoshikawa T, Ishikawa Y, Shibuya K, Okamura N, Iwata R, Yanai K.
    • Journal Title

      Nucl Med Biol

      Volume: 39(1) Pages: 155-160

    • Peer Reviewed
  • [Presentation] 無細胞蛋白質合成系を用いた^<11>C標識一本鎖抗体の合成2012

    • Author(s)
      伊藤悠一、古本祥三、松田貴意、樋口佳恵、横山順、張明栄、谷内一彦、岩田錬、木川隆則
    • Organizer
      第52回日本核医学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      2012-10-11

URL: 

Published: 2014-09-25  

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