2012 Fiscal Year Final Research Report
Novel synthetic approach for a positron emitter labeled protein by cell free protein synthesis system
| Project/Area Number |
23791387
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Tohoku University |
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Principal Investigator |
FURUMOTO Shozo 東北大学, 大学院医学系研究科, 准教授 (00375198)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 放射性医薬品 / 造影剤 |
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| Research Abstract |
In this study, we evaluated a novel synthetic approach for a positron emitter labeled protein by cell-free protein synthesis (CFPS) system. Synthesis of ^<11>C-GFP using ^<11>C-Met and CFPS afforded the product within a short time. On the other hand, when using ^<18>F-Pro, the ^<18>F-labeled GFP was produced too slowly to use it practically. We succeeded to prepare^<11>C-labeled scFv antibody to EGFR, which showed specific binding to EGFR-positive tumorcells in vitro and specific uptake by the EGFR-positive tumor in vivo. These results suggest the usefulness of the ^<11>C-labeld protein synthesis by CFPS.
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