2013 Fiscal Year Final Research Report
Development of a novel site-specific 18F protein labeling method and application for synthesis of 18F-EGF
| Project/Area Number |
23791465
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Kumamoto University (2012-2013)
The Institute of Physical and Chemical Research (2011) |
|---|
Principal Investigator |
HASEGAWA Koki 熊本大学, 生命科学研究部, 助教 (50525798)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | EGF / 上皮性腫瘍 / SPECT / 67Ga / 分子イメージング |
|---|
| Research Abstract |
We prepared NODAGA-EGF as a molecular probe and set up the molecular imaging device in Kumamoto University. EGF, which consists of 53 amino acid residues, was synthesized by Fmoc solid-phase method. After elongation of peptide chain, NODAGA(tBu)3 was introduced at N-terminus. Fully protected peptide resin was treated in TFA solution to cleave from resin and remove protection groups. After HPLC purification, disulfide bonds were formed to give the labeling precursor. Next, to perform molecular imaging study, SPECT/CT was set up. We established a method to form stable complex with Ga-67 via DOTA chelator. SPECT device was checked the accuracy of quantification and image resolution. SPECT imaging using 67Ga-DOTA-octreotate was carried out on the mouse implanted with small cell lung tumor cell (H69). As the result, we could observe the accumulation of the Ga-67 in H69 tumor.
|
-
[Journal Article] Positron emission tomography study on pancreatic somatostatin receptors in normal and diabetic rats with ^<68>Ga-DOTA-octreotide : A potential PET tracer for beta cell mass measurement (2013)2013
Author(s)
Sako, T., Hasegawa, K., Nishimura, M., Kanayama, Y., Wada, Y., Hayashinaka, E., Cui, Y., Kataoka, Y., Senda, M., Watanabe, Y.
-
Journal Title
Biochemical and Biophysical Research Communications
Volume: 442 (1-2)
Pages: pp. 79-84
Peer Reviewed
-
[Journal Article] Novel molecular imaging of atherosclerosis with Gallium-68-labeled Apolipoprotein A-I mimetic peptide and positron emission Tomography (2013)2013
Author(s)
Kawachi, E., Uehara, Y., Hasegawa, K., Yahiro, E., Ando, S., Wada, Y., Yano, T., Nishikawa, H., Shiomi, M., Miura, S., Watanabe, Y., Saku, K.
-
Journal Title
Circulation Journal
Volume: 77 (6)
Pages: pp. 1482-1489
Peer Reviewed
-
[Journal Article] Detection of early stage atherosclerotic plaques using PET and CT fusion imaging targeting P-selectin in low density lipoprotein receptor-deficient mice (2013)2013
Author(s)
Nakamura, I., Hasegawa, K., Wada, Y., Hirase, T., Node, K., Watanabe, Y.
-
Journal Title
Biochemical and Biophysical Research Communications
Volume: 433 (1)
Pages: pp. 47-51
Peer Reviewed
-
-
-
-
-
