Approach of radiochemistry about cellular lethality in DNA repair-deficient cell lines after heavy-ion irradiation

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791472
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: HIRAYAMA Ryoichi 独立行政法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (90435701)
• Project Period (FY): 2011 – 2013
• Keywords: 放射線治療生物学 / 重粒子線 / 生物学的効果比 / 酸素増感比 / 低酸素 / OHラジカル / 陽子線 / DNA二本鎖切断修復

Research Abstract

We estimated the effects of indirect and direct actions on cell killing using dimethylsulfoxide (DMSO). CHO, xrs6 and 51D1 cell lines under oxic and hypoxic conditions were exposed to several intensities of high-LET radiation (13 to 200 keV/micrometer) in the presence or absence of DMSO and cell survival was determined using a colony formation assay. Under both oxic and hypoxic conditions, the contribution of direct action to cell killing increased with increasing LET. In all cell lines, the RBEs determined at a survival level of 10% increased with LET, and the OERs decreased with LET. When the RBE and OER were estimated separately for direct action (RBE-D and OER-D) and indirect action (RBE-I and OER-I); the RBE-D was larger than RBE-I and the OER-D was smaller than OER-I. Thus the direct action of heavy-ions yielded a remarkably larger RBE and smaller OER for cell killing than the indirect action.

Research Products

• [Journal Article] OH radicals from the indirect actions of X-rays induce cell lethality and mediate the majority of the oxygen enhancement effect (2013)
  • Author(s): Ryoichi Hirayama, Atsushi Ito, Miho Noguchi, Yoshitaka Matsumoto, Akiko Uzawa, Gen Kobashi, Ryuichi Okayasu, Yoshiya Furusawa
  • Journal Title: Radiation Research Volume: 180 Pages: 514-523
  • DOI: 10.1667/RR13368.1
  • Peer Reviewed
• [Presentation] 高LET放射線とフリーラジカルの生物影響 (2013)
  • Author(s): 平山亮一、松本孔貴、鵜澤玲子、金子由美子、平野祥之、尾崎匡邦、山下慶、李惠子、苅谷文子、野口実穂、白井敏之、古澤佳也
  • Organizer: 日本放射線影響学会第56回大会
  • Place of Presentation: 青森
  • Year and Date: 2013-10-19
• [Presentation] X線ならびに重粒子線の酸素効果 (2013)
  • Author(s): 平山亮一、松本孔貴、鵜澤玲子、金子由美子、尾崎匡邦、山下慶、李惠子、苅谷文子、野口実穂、白井敏之、古澤佳也
  • Organizer: 第42回放射線治療による制癌シンポジウム-基礎と臨床の対話-
  • Place of Presentation: 仙台
  • Year and Date: 2013-07-05
• [Presentation] 重粒子線誘発フリーラジカルの生物影響 (2013)
  • Author(s): 平山亮一、古澤佳也
  • Organizer: 第6回光・プラズマプロセスのバイオ応用ワークショップ
  • Place of Presentation: 大阪
  • Year and Date: 2013-03-08
• [Presentation] 重粒子線の放射線生物学-3次元スキャニング照射法による生物学的特徴- (2013)
  • Author(s): 平山亮一、古澤佳也
  • Organizer: 第11回九州放射線治療システム研究会
  • Place of Presentation: 福岡
  • Year and Date: 2013-01-19
• [Presentation] High biological effectiveness depends on the direct action by heavy ions (2012)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Kana Koda, Akiko Uzawa, Miho Noguchi, Yuki Kase, Atsushi Ito, Naruhiro Matsufuji, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: 39th Annual Meeting of the European Radiation Research Society
  • Place of Presentation: Vietri sul Mare, Italy
  • Year and Date: 2012-10-18
• [Presentation] 酸素効果のメカニズムにおける化学的因子と生物学的因子 (2012)
  • Author(s): 平山亮一、伊藤敦、安藤興一、古澤佳也
  • Organizer: 日本放射線影響学会第55回大会
  • Place of Presentation: 仙台
  • Year and Date: 2012-09-06
• [Presentation] 水の放射線分解によって生じたフリーラジカルの生物影響 (2012)
  • Author(s): 平山亮一
  • Organizer: 平成24年度第1回量子ビーム科学研究施設研究会
  • Place of Presentation: 茨木市
  • Year and Date: 2012-07-25
• [Presentation] 3次元スキャニング照射法による生物学的利点 (2012)
  • Author(s): 平山亮一、松本孔貴、鵜澤玲子、幸田華奈、野口実穂、加瀬優紀、松藤成弘、伊藤敦、古澤佳也
  • Organizer: 第50回日本放射線腫瘍学会生物部会学術大会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-06-30
• [Presentation] Direct action of heavy-ion plays a major role in the high biological effects (2012)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Kana Koda, Akiko Uzawa, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Atsushi Ito, Yoshiya Furusawa
  • Organizer: 12th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2012-06-06
• [Presentation] 重粒子線の直接作用がもたらす生物効果 (2012)
  • Author(s): 平山亮一、松本孔貴、鵜澤玲子、幸田華奈、野口実穂、加瀬優紀、松藤成弘、伊藤敦、古澤佳也
  • Organizer: 第14回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良
  • Year and Date: 2012-02-11
• [Presentation] X線ならびに高LET放射線によって生成されたDNA損傷の低酸素環境下での修復動態 (2011)
  • Author(s): 平山亮一、松本孔貴、野口実穂、鵜澤玲子、幸田華奈、古澤佳也
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-18
• [Presentation] Direct action is the key to understand the high biological effects caused by particle beams (2011)
  • Author(s): Ryoichi Hirayama, Yoshitaka Matsumoto, Akiko Uzawa, Nobuhiro Takase, Chizuru Tsuruoka, Mami Wada, Miho Noguchi, Yuki Kase, Naruhiro Matsufuji, Atsushi Ito, Ryuichi Okayasu, Yoshiya Furusawa
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2011-08-30