Research of hepatocyte transplantation for injured liver and surgical stress .

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791489
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Sapporo Medical University
• Principal Investigator: NAKAMURA YUKIO 札幌医科大学, 医学部, 研究員 (50516648)
• Project Period (FY): 2011 – 2012
• Keywords: 肝臓外科学

Research Abstract

In recently, surgical management of hepatocellular carcinoma should be addressed for non-viral hepatitis and liver cirrhosis. We attempt to apply liver replacement therapy using hepatocyte transplantation for clinical therapy. In order to achieve it, we developed rat liver replacement model which imitated clinical human NASH/NAFLD. In this model, we revealed a cell physiology of the transplanted cells which generated bile duct linage cells. In addition, we succeded to increase survival rate in NASH liver cirrhotic model. In the clinical research, we reported a benefit and default of donor cell procurement. We also revealed that a clinical value of BTR, ATIII, and GSA. We classified various liver functional indicators using clustering analysis.

Research Products

• [Journal Article] New technique of extracorponeal hepatic inflow control for pure laparoscopic liver resection (2013)
  • Author(s): Mizuguchi T, Kawamoto M, Nakamura Y, Meguro M, Harada K, OtaS, Hui TT, and Hirata K.
  • Journal Title: Surg Laparosc Endosc Percutan Tech Volume: (in press)
  • Peer Reviewed
• [Journal Article] Differentiation capacity of hepatic stem/progenitor cells isolated from D-galactOsamine-treated rat livers (2013)
  • Author(s): Ichinohe N, Tanimizu N, Ooe H, Nakamura Y, Mizuguchi T, Kon J, Hirata K, Mitaka T.
  • Journal Title: Hepatology Volume: 57 Pages: 1192-202
  • DOI: 10.1002/hep.26084
  • Peer Reviewed
• [Journal Article] Hepatic biliary epithelial cells acquire epithelial integrity but lose plasticity to differentiate into hepatocytes in vitro during development (2013)
  • Author(s): Tanimizu N, Nakamura Y, Ichinohe N, Mizuguchi T, Hirata K, Mitaka T.
  • Journal Title: J Cell Sci Volume: 126 Pages: 5239-46
  • DOI: 10.1242/jcs.133082
  • Peer Reviewed
• [Journal Article] Prognosis and Predictors of Surgical Complications in Hepatocellular Carcinoma Patients With or Without Cirrhosis after Hepatectomy (2013)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Nakamura Y, Ota S, Hui TT, Hirata K.
  • Journal Title: World J Surg Volume: 37 Pages: 1379-87
  • DOI: 10.1007/s00268-013-1989-6
  • Peer Reviewed
• [Journal Article] Continuous monitoring of central venous oxygen saturation predicts postoperative liver dysfunction after liver resection (2013)
  • Author(s): Meguro M, Mizuguchi T, Kawamoto M, Nakamura Y, Ota S, Kukita K, Ishii M, Tatsumi H, Hirata K.
  • Journal Title: Surgery Volume: 154 Pages: 351-62
  • DOI: 10.1016/j.surg.2013.04.039
  • Peer Reviewed
• [Journal Article] Preoperative hepatocyte transplantation improves the survival of rats with non -alcoholic steatohepatitis-related cirrhosis after partial hepatectomy (2013)
  • Author(s): Nakamura Y, Mizuguchi T, Tanimizu N, Ichinohe N, Ooe H, Kawamoto M, Meguro M, Hirata K, Mitaka T.
  • Journal Title: Cell Transplant Volume: Jun 13
  • DOI: 10.3727/096368913X668649
  • Peer Reviewed
• [Journal Article] Serum antithrombin III level is well correlated with multiple indicators for assessment of liver function and diagnostic accuracy for predicting postoperative liver failure in hepatocellular carcinoma patients (2012)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Son S, Nakamura Y, Harada K, Shibata T, Ota S, Hirata K.
  • Journal Title: Hepatogastroenterology Volume: 59 Pages: 551-7
  • DOI: 10.5754/hge10052
  • Peer Reviewed
• [Journal Article] Low dose steroid pretreatment ameliorates transient impairment of the liver regeneration due to radiofrequency ablation (2012)
  • Author(s): Shibata T, Mizuguchi T, Nakamura Y, Kawamoto M, Meguro M, Ota S, Hirata K, Ooe H, Mitaka T.
  • Journal Title: World J Gastroenterol Volume: 18 Pages: 905-14
  • DOI: 10.3748/wjg.v18.i9.905
  • Peer Reviewed
• [Journal Article] Laparoscopic hepatectomy : a systematic review, meta-analysis and power analysis (2011)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Shibata T, Nakamura Y, Kimura Y, Furuhata T, Sonoda T, Hirata K.
  • Journal Title: Surg Today Volume: 41 Pages: 39-47
  • DOI: 10.1007/s00595-010-4337-6
  • Peer Reviewed
• [Journal Article] Prognostic impact of preoperative branched-chain amino acids to the tyrosine ratio level in hepatocellular carcinoma patients after initial hepatectomy (2011)
  • Author(s): Mizuguchi T, Kawamoto M, Meguro M, Nakamura Y, Harada K, Kukita K, Hirata K.
  • Journal Title: J Gastroint Surg Volume: 15 Pages: 1433-9
  • DOI: 10.1007/s11605-011-1566-y
  • Peer Reviewed
• [Journal Article] Cluster analysis of liver functional indicators and preoperative low BTR indicate high risk of early recurrence in analysis of 165 HCC patients after initial hepatectomy (2011)
  • Author(s): Nakamura Y, Mizuguchi T, Kawamoto M, Meguro M, Harada K, Ota S, Hirata K.
  • Journal Title: Surgery Volume: 150 Pages: 250-62
  • DOI: 10.1016/j.surg.2011.06.001
  • Peer Reviewed
• [Presentation] Continuous monitoring of central venous oxygen saturation predicts postoperative liver dysfunction after liver resection (2013)
  • Author(s): Meguro M, Mizuguch T, Kawamoto M, Nakamura Y, Kukita K, Ota S, Hirata K.
  • Organizer: 8th Annual Academic Surgical Congress, Oral presentation
  • Place of Presentation: New Orleans, LO, USA
  • Year and Date: 20130205-07
• [Presentation] Hepatic Progenitor Cell Transplantation for Improving Survival after Liver Resection of Rat Non-alcoholic Steatocirrhotic Liver Model (2012)
  • Author(s): Nakamura Y, Mizuguchi T, Ohe H, Kawamoto K, Meguro M, Ota S, Mitaka T, Hirata K.
  • Organizer: 7th Annual Academic Surgical Congress, Quick shot oral presentation
  • Place of Presentation: Las Vegas, NV, USA
  • Year and Date: 20120214-16
• [Presentation] Extracorporeal hepatic inflow control method for pure laparoscopic liver resection (2012)
  • Author(s): Mizuguchi T, Kawamoto K, Meguro M, Nakamura Y, Harada K, Nobuoka T, Kimura Y, Furuhata T, Hirata K.
  • Organizer: 7th Annual Academic Surgical Congress, Quick shot oral presentation
  • Place of Presentation: Las Vegas, NV, USA
  • Year and Date: 20120214-16