2013 Fiscal Year Final Research Report
Cancer vaccine therapy using genetically modified induced pluripotent stem cell-derived dendritic cells expressing the TAA gene.
| Project/Area Number |
23791492
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 樹状細胞 / iPS細胞 / 癌ワクチン |
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| Research Abstract |
We developed the induced pluripotent stem cell-derived dendritic cells DCs (iPSDCs) from iPS cells and examined the capacity for maturation of iPSDCs compared to that of bone marrow-derived DCs (BMDCs) in addition to the capacity for migration of iPSDCs to lymph nodes. We adenovirally transduced the hgp100 gene, natural tumor antigens, into DCs and immunized mice once with the genetically modified DCs. Our results showed that iPSDCs have an equal capacity to BMDCs in terms of maturation and migration. Furthermore, hgp100-specific CTLs were generated in mice immunized with genetically modified iPSDCs. These CTLs exhibited as high a level of cytotoxicity against B16 cells as BMDCs. Moreover, vaccination with the genetically modified iPSDCs achieved as high a level of therapeutic efficacy as vaccination with BMDCs. Our study clarified that genetically modified iPSDCs have an equal capacity to BMDCs in terms of tumor-associated antigen specific therapeutic antitumor immunity.
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