Hepatocellular Telomere Length in Biliary Atresia

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791495
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Jichi Medical University
• Principal Investigator: SANADA Yukihiro 自治医科大学, 医学部, 助教 (60406113)
• Project Period (FY): 2011 – 2013
• Keywords: テロメア / 胆道閉鎖症 / 肝移植 / Q-FISH法 / Southern blot法 / PELDスコア

Research Abstract

As the native liver of biliary atresia patients is cirrhotic and the long-term survival rate of such patients with their native liver is low, coupled with the fact that extremely short telomeres are associated with subsequent carcinogenesis and organ failure, it can be said that prominent telomere shortening in the hepatic tissue of biliary atresia patients in comparison with normal controls supports the early implementation of liver transplantation, regardless of the degree of liver cirrhosis.
Telomere length analysis using quantitative fluorescence in situ hybridization could be an objective indicator of hepatocellular reserve capacity in patients with biliary atresia, and excessive telomere shortening supports the early implementation of liver transplantation.

Research Products

• [Journal Article] Hepatocellular Telomere Length in Biliary Atresia Measured by Q-FISH (2012)
  • Author(s): Sanada Y, Aida J, Kawano Y, Nakamura K, Shimomura N, Ishikawa N, Arai T, Poon SS, Yamada N, Okada N, Wakiya T, Hayashida M, Saito T, Egami S, Hishikawa S, Ihara Y, Urahashi T, Mizuta K, Yasuda Y, Kawarasaki H, Takubo K.
  • Journal Title: World J Surg Volume: Apr;36(4) Pages: 908-16
  • DOI: 10.1007/s00268-012-1453-z
• [Presentation] 胆道閉鎖症の肝組織における細胞老化関連マーカーの解析 (2013)
  • Author(s): 眞田幸弘,浦橋泰然,井原欣幸,脇屋太一,岡田憲樹,山田直也,平田雄大,水田耕一
  • Organizer: 第40回日本胆道閉鎖症研究会
  • Place of Presentation: 茨城県水戸市
  • Year and Date: 2013-11-16
• [Presentation] 胆道閉鎖症の肝組織における細胞老化関連β-galactosidaseの解析 (2012)
  • Author(s): 眞田幸弘,相田順子,川野陽一,下村七生貴,仲村賢一,石川直,新井冨生,山田直也,岡田憲樹,脇屋太一,井原欣幸,浦橋泰然,水田耕一,田久保海誉
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2012-04-28
• [Presentation] 胆道閉鎖症の肝組織におけるテロメア長の解析 (2011)
  • Author(s): 眞田幸弘,相田順子,川野陽一,仲村賢一,泉山-下村七生貴,石川直,山田直也,岡田憲樹,脇屋太一,井原欣幸,江上聡,菱川修司,浦橋泰然,水田耕一,河原﨑秀雄,田久保海誉
  • Organizer: 第10回自治医科大学シンポジウム
  • Place of Presentation: 栃木県下野市
  • Year and Date: 2011-09-02