2012 Fiscal Year Final Research Report
Early Postoperative application of extracorporeal leukocyte apheresis in patients with crohn's disease to prevent postoperative septic complications
| Project/Area Number |
23791523
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Mie University |
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Principal Investigator |
YOSHIYAMA Shigeyuki 三重大学, 大学院・医学系研究科, リサーチアソシエイト (60444436)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 小腸大腸肛門外科学 |
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| Research Abstract |
We previously reported that ulcerative colitis (UC) patients had excessively activated neutrophils which were less apoptotic and more necrotic by co-cultured with Escherichia coli (E. coli). Postoperative leukocyte removal therapy (leukocytapheresis: LCAP) reduced the rate of surgical site infection (SSI) in UC patients. LCAP may inhibit excessive immune response due to excessively activated neutrophils. In this study, we examine the induction rate of viable neutrophils, apoptotic cells, necrotic cells and secondary necrotic cells after co-culture with E. coli to explore the functional profile of neutrophils of Crohn’s disease (CD) patients. The induction rate of apoptotic cells after co-culture with E. coli was significantly lower in CD patients than that of control, suggesting that neutrophils of CD patients are likely to suppress apoptosis induction when exposed by bacteria. In contrast, the induction rate of necrotic cells after co-culture with E. coli was significantly higher in CD patients than that of control, suggesting that neutrophils of CD patients are likely to induce necrosis when exposed by bacteria. Preoperative LCAP significantly increased the ratio of apoptosis to necrosis after co-culture with E. coli in UC patients. Postoperative LCAP significantly decreased the rate of necrosis after co-culture with E. coli in UC patients.These results suggested that LCAP may remove the neutrophils which are likely to induce necrosis when exposed by bacteria. The cDNA microarray analysis showed that neutrophils obtained from UC patients had significantly higher expression in apoptosis related genes such as OPA1, SH3GLB1, BCL2A, BNIP2, and BIRC1.
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Research Products
(16 results)
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[Journal Article] CD133, OCT4, and NANOG in ulcerative colitis-associated colorectal cancer2011
Author(s)
Yasuda H, Tanaka K, Okita Y, Araki T, Saigusa S, Toiyama Y, Yokoe T, Yoshiyama S, Kawamoto A, Inoue Y, Miki C, Kusunoki M
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Journal Title
Oncol Lett
Volume: 2
Pages: 1065-1071
Peer Reviewed
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[Presentation] 小児期クローン病に対するダブルバルーン内視鏡施行の意義2012
Author(s)
井上 幹大, 大北 喜基, 井出 正造, 橋本 清, 安田裕美, 藤川 裕之, 小池 勇樹, 吉山 繁幸, 荒木 俊光, 内田 恵一, 楠 正人
Organizer
第54回日本消化器病学会大会
Place of Presentation
神戸
Year and Date
20121010-13
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[Presentation] インフリキシマブ登場後のIBDに対する手術療法 手術のタイミングとその術式 潰瘍性大腸炎術前治療薬が術後合併症に与える影響の検討2011
Author(s)
荒木 俊光, 吉山 繁幸, 大北 喜基, 藤川 裕之, 川村幹雄, 近藤 哲, 小池 勇樹, 井上 幹大, 問山 裕二, 大井 正貴, 井上 靖浩, 田中 光司, 内田 恵一, 毛利 靖彦, 楠正人
Organizer
第73回日本臨床外科学会総会
Place of Presentation
東京
Year and Date
20111117-19
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[Presentation] 梨状筋膿瘍を合併したクローン病の1例2011
Author(s)
押 正徳, 荒木 俊光, 吉山 繁幸, 大北喜基, 藤川 裕之, 川村 幹雄, 問山 裕二, 大井 正貴, 田中 光司, 井上 靖浩, 内田 恵一, 毛利 靖彦, 楠 正人
Organizer
第73回日本臨床外科学会総会
Place of Presentation
東京
Year and Date
20111117-19
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[Presentation] クローン病術後再手術抑制に対するインフリキシマブ維持投与の影響2011
Author(s)
荒木俊光, 吉山 繁幸, 大北 喜基, 藤川 裕之, 川村 幹雄, 沖上 正人, 北嶋 貴仁, 松下 航平, 大竹 耕平, 井上 幹大, 内田 恵一, 三木 誓雄, 楠 正人
Organizer
第111回日本外科学会定期学術集会
Place of Presentation
東京
Year and Date
20110400
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[Presentation] 小児潰瘍性大腸炎手術例におけるQOL評価2011
Author(s)
内田 恵一, 井上 幹大, 大竹 耕平, 荒木 俊光, 吉山 繁幸, 大北 喜基, 松下 航平, 橋本 清, 田中 光司, 三木誓雄, 楠 正人
Organizer
第111回日本外科学会定期学術集会
Place of Presentation
東京
Year and Date
20110400
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[Presentation] 小児に対する小腸ダブルバルーン内視鏡施行症例の検討2011
Author(s)
井上 幹大, 内田恵一, 吉山 繁幸, 井出 正造, 橋本 清, 松下 航平, 安田 裕美, 小池 勇樹, 大北 喜基, 大竹 耕平, 荒木 俊光, 楠正人
Organizer
第38回日本小児内視鏡研究会
Place of Presentation
軽井沢
Year and Date
2011-07-02
