Investigation of the lymph node metastasis mechanism throughout niche around cancer stem cell in esophageal squamous cell carcinoma

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Kagoshima University
• Principal Investigator: KITA Yoshiaki 鹿児島大学, 医学部・歯学部附属病院, 特任助教 (30570692)
• Project Period (FY): 2011 – 2012
• Keywords: 微小環境 / 癌幹細胞 / マイクロアレイ / 細胞実験

Research Abstract

We found some genes regarding as niche of cancer stem cell by using oligomicroarray with laser micro dissection method. (1) One of above genes, which concerns cell cycle, was evaluated the expression and function and associated with invasion of tumor, lymphatic invasion, clinical stage, distant metastasis, and prognosis. (2) We extract Glut-1 which is regarding glucose metabolism, and evaluated the expression of the cell membrane of tumor cell. After we consider the result of PET-CT which is thediagnostic modality using glucose uptake into the tumor cell, we investigated that Glut-1expression has correlate with FDG accumulation. Finally, we submitted this result to Annals of Surgical Oncology and they accepted as follows.

Research Products

• [Journal Article] Sox2 cooperates with inflammation-mediated stat3 activation in the malignant transformation of foregut Basal progenitor cells (2013)
  • Author(s): Liu K, Jiang M, Lu Y, Chen H, Sun J, Wu S, Ku WY, Nakagawa H, Kita Y, Natsugoe S, Peters JH, Rustgi A, Onaitis MW, Kiernan A, Chen X, Que J
  • Journal Title: Cell Stem Cell Volume: 12(3) Pages: 304-15
  • Peer Reviewed
• [Journal Article] Clinical significance of 18F-fluorodeoxyglucose position emission tomography in superficial esophageal squamous cell carcinoma (2013)
  • Author(s): Kita Y, Okumura H, Uchikado Y, Sasaki K, Omoto I, Matsumoto M, Setoyama T, Tanoue K, Mori S, Owaki T, Ishigami S, Ueno S, Kajiya Y, Natsugoe S
  • Journal Title: Ann Surg Oncol Volume: 20(5) Pages: 1646-52
  • DOI: DOI:10.1245/s10434-012-2796-2
  • Peer Reviewed
• [Journal Article] 1 期的縫合閉鎖が有効であった食道穿孔の 2 例.臨床外科 (2012)
  • Author(s): 喜多芳昭,松本正隆,内門泰斗,奥村浩,大脇哲洋,夏越祥次
  • Journal Title: 医学書院 Volume: 67(5) Pages: 715-719
  • URL: http://www.bitway.ne.jp/ejournal/so-net/1407104089.html
  • Peer Reviewed
• [Journal Article] 緩やかな経過をとり術前診断が困難であった絞扼性イレウスの1例.外科 (2011)
  • Author(s): 喜多芳昭,宮薗太志,萩原貴彦,才原哲史,夏越祥次
  • Journal Title: 南江堂 Volume: 73(1) Pages: 101-104
  • URL: http://ci.nii.ac.jp/naid/40017648714
  • Peer Reviewed
• [Presentation] 切除不能局所進行食道癌に対する治療方針と予後の検討 (2012)
  • Author(s): 喜多芳昭,奥村浩,内門泰斗,佐々木健,田上聖徳,松本正隆,平木嘉幸,大脇哲洋,石神純也,夏越祥次
  • Organizer: 第66回日本食道(ポスター)
  • Place of Presentation: 所沢市
  • Year and Date: 2012-06-22
• [Presentation] 大腸癌 Sentinel Node 理論の臨床応用の可能性 (2012)
  • Author(s): 喜多芳昭,上之園芳一,有上貴明,有馬豪男,萩原貴彦,原口尚士,盛真一郎,北薗正樹,石神純也,上野真一,夏越祥次
  • Organizer: 第112回日本外科学会定期学術総会(サージカルフォーラム)
  • Place of Presentation: 幕張
  • Year and Date: 2012-04-12
• [Presentation] 腹腔鏡補助下に切除した横行結腸脂肪腫の 1 例 (2011)
  • Author(s): 喜多芳昭,山崎洋一,北薗正樹,内門泰斗,有上貴明,松本正隆,奥村浩,大脇哲洋,石神純也,上野真一,夏越祥次
  • Organizer: 第24回日本内視鏡外科学会総会(一般演題)
  • Place of Presentation: 大阪
  • Year and Date: 2011-12-09
• [Presentation] 下部直腸癌に対して術前化学放射線療法を併用し肛門温存手術を行った 2 例 (2011)
  • Author(s): 喜多芳昭,盛真一郎,内門泰斗,有上貴明,北薗正樹,石神純也,上野真一,夏越祥次
  • Organizer: 第36回大腸肛門病学会九州地方会(一般演題)
  • Place of Presentation: 福岡市
  • Year and Date: 2011-11-05
• [Presentation] 食道表在癌における FDG-PET の有用性 (2011)
  • Author(s): 喜多芳昭,松本正隆,内門泰斗,奥村 浩,尾本 至,田上聖徳,瀬戸山徹郎,大脇哲洋,石神純也,上野真一,夏越祥次
  • Organizer: 第9回消化器外科学会大会(JDDW) (ポスター)
  • Place of Presentation: 福岡市
  • Year and Date: 2011-10-23
• [Presentation] Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma (2011)
  • Author(s): 喜多芳昭,松本正隆, 内門泰斗,瀬戸山徹郎,田上聖徳,大脇哲洋,奥村 浩,石神純也,新地洋之,上野真一,三森功士,森 正樹,夏越祥次
  • Organizer: 第70回日本癌学会総会(ポスター)
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-03
• [Presentation] Expression of uPAR mRNA in peripheral blood is a favourite marker for metastasis in gastric cancer cases (2011)
  • Author(s): Yoshiaki Kita, Masataka Matsumoto, Hiroshi Okumura, Yasuto Uchikado, Tetsuro Setoyama, Sumiya Ishigami, Tetsuhiro Owaki, Koshi Mimori, Takeo Fukagawa,Masaki Mori, Shoji Natsugoe
  • Organizer: 8th International Symposium on Minimal Residual Cancer (Oral presentation)
  • Place of Presentation: Osaka
  • Year and Date: 2011-09-21
• [Presentation] 腹腔鏡下に腹壁瘢痕ヘルニア根治術を行った 2 例(一般演題) (2011)
  • Author(s): 喜多芳昭,貴島孝,内門泰斗,北薗正樹,石神純也,上野真一,夏越祥次
  • Organizer: 第21回九州内視鏡下外科手術研究会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-09-03
• [Presentation] 食道表在癌における FDG-PET の意義 (2011)
  • Author(s): 喜多芳昭,松本正隆,内門泰斗,奥村 浩,尾本 至,田上聖徳,瀬戸山徹郎,大脇哲洋,石神純也,加治屋より子,夏越祥次
  • Organizer: 第66回消化器外科学会総会(企画関連口演)
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-07-13
• [Presentation] 食道扁平上皮癌における LAMB3 と COL7A1 の発現
  • Author(s): 喜多芳昭,松本正隆,内門泰斗,奥村 浩,瀬戸山徹郎,大脇哲洋,石神純也,三森功士,森 正樹,夏越祥次
  • Organizer: 第65回食道学会総会
  • Place of Presentation: 紙面発表
• [Presentation] 食道疾患における食道洗浄液培養の検討
  • Author(s): 喜多芳昭,大脇哲洋,松本正隆,内門泰斗,尾本 至,恵 浩一,奥村 浩,瀬戸山徹郎,石神純也,新地洋之,上野真一,夏越祥次
  • Organizer: 第111回日本外科学会定期学術総会
  • Place of Presentation: 紙面発表
• [Book] 癌転移の分子機構.消化器外科学レビュー2012 -最新主要文献と解説- (2012)
  • Author(s): 喜多芳昭,夏越祥次
  • Total Pages: 197-202
  • Publisher: 総合医学社
• [Book] 癌の遺伝子診断・治療.消化器外科学レビュー2011 -最新主要文献と解説- (2011)
  • Author(s): 喜多芳昭,夏越祥次
  • Total Pages: 195-200
  • Publisher: 総合医学社