2012 Fiscal Year Final Research Report
Optimization of collagenase activity and regulation of ion channel associated with cell viability for improving pancreatic islet transplantation
| Project/Area Number |
23791554
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 膵島移植 / 膵島分離 / 糖尿病 / イオンチャネル |
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| Research Abstract |
Protection of pancreatic islets during isolation procedures andmaximization of islet yield are mandatory for successful islet transplantation. An enzyme mixture composed of neutral protease with high intact class I collagenase recovered higher islet yield from both rat and human pancreas. Our results also indicated that Clinflux into the cells causes the cell damage during the digestion procedure. Inhibition of Clinflux into islets by Clchannel inhibitor or Cl--free condition protects islets in both heart-beating and non-heart-beating donor model, offering a new strategy for improving human islet isolation outcome.
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Research Products
(13 results)
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[Journal Article] A new enzyme mixture to increase the yield and transplant rate of autologous and allogeneic human islet products2012
Author(s)
Balamurugan AN, Loganathan G, Bellin MD, Wilhelm JJ, Harmon J, Anazawa T, Soltani SM, Radosevich DM, Yuasa T, Tiwari M, Papas KK, McCarthy R, Sutherland DE, Hering BJ
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Journal Title
Transplantation
Volume: 93(7)
Pages: 693-702
DOI
Peer Reviewed
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[Journal Article] Improved islet yield and function by use of a chloride channel blocker during collagenase digestion2011
Author(s)
Anazawa T, Sato Y, Saito T, Tsuchiya T, Kenjo A, Kimura T, Haga J, Miyake M, Waguri S, Hazama A, Gotoh M
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Journal Title
Transplantation
Volume: 92(8)
Pages: 871-877
DOI
Peer Reviewed
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[Presentation] Disappeared islet graft after intraportal transplantation in the recipient suffered from recurrent sepsis2012
Author(s)
Anazawa T, Tsuchiya T, Kenjo A, Haga J, Sato T, Sato N, Ise K, Saito T,Tasaki K, Gotoh M
Organizer
24th International Congress of the Transplantation Society
Place of Presentation
Berlin, Germany
Year and Date
20120715-19
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[Presentation] Inhibition of chloride ion influx into islet cells protects islets during pancreatic islet isolation2011
Author(s)
Anazawa T, Tsuchiya T, Kenjo A, Haga J, Sato T, Sato N, Saito T, Sato Y, Miyake M, Hazama A, Gotoh M
Organizer
The 13th World congress of International pancreas and islet transplantation association
Place of Presentation
Prague, Czech Republic
Year and Date
20110601-04
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[Presentation] Mitomycin-C treatment protects islets from the progression of central damage during culture.2011
Author(s)
Haga J, Saito T, Anazawa T, Tsuchiya T, Kenjo A, Sato S, Sato N, Saito T, Ise K, Yamamoto M, Waguri S, Gotoh M
Organizer
The 13th World congress of International pancreas and islet transplantation association
Place of Presentation
Prague, Czech Republic
Year and Date
20110601-04
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