Production of anti-mutated IDH1/2 monoclonal antibodies and its clinical application

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791584
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Yamagata University
• Principal Investigator: KATO Yukinari 山形大学, 医学部, 非常勤講師 (00571811)
• Project Period (FY): 2011 – 2012
• Keywords: モノクローナル抗体 / グリオーマ / IDH1 / IDH2 / 変異型IDH1/2 / R132H / R132S / 免疫組織染色

Research Abstract

Isocitrate dehydrogenase IDHmutations occur in low-grade gliomas. However, specific mAbs against mutated IDH1 have not been reported. In this study, we established anti-mutated IDH1-specific mAbs HMab-1 and SMab-1, which are useful in immunohistochemistry of glioma.

Research Products

• [Journal Article] Immunohistochemical analysis-based proteomic subclassification of newly diagnosed glioblastomas with integrated genomic and epigenetic profiles (2012)
  • Author(s): Motomura K, Natsume A, Watanabe R, Ito I, Kato Y, Momota H, Nishikawa R, Mishima K, Nakasu Y, Abe T, Namba H, Nakazato Y, Tashiro H, Takeuchi I, Mori T, Wakabayash T
  • Journal Title: Cancer Sci Volume: 103(10) Pages: 1871-9
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of IDH1 mutation, p53 and internexin as a prognostic factor of glial tumors (2012)
  • Author(s): Takano S, Kato Y, Yamamoto T, Kaneko MK, Ishikawa E, Tsujimoto Y, Matsuda M, Nakai K, Yanagiya R, Morita S, Tsuboi K, Matsumura A
  • Journal Title: J Neurooncol. Volume: 108(3) Pages: 361-73
  • Peer Reviewed
• [Journal Article] IDH1 mutation is a favorable prognostic factor for type 2 gliomatosis cerebri (2012)
  • Author(s): Kwon MJ, Kim ST, Kwon MJ, Kong DS, Lee D, Park S, Kang SY, Song JY, Nam DH, Kato Y, Choi YL, Suh YL
  • Journal Title: Brain Pathol Volume: 22(3) Pages: 307-317
  • Peer Reviewed
• [Journal Article] Establishment of a novel monoclonal antibody SMab-1 specific for IDH1 R132S mutation (2011)
  • Author(s): Kaneko MK, Tian W, Takano S, Suzuki H, Sawa Y, Hozumi Y, Goto K, Yamazaki K, Kato Y
  • Journal Title: Biochem Biophys Res Commun Volume: 406(4) Pages: 608-613
  • Peer Reviewed
• [Journal Article] Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing (2011)
  • Author(s): Takano S, Tian W, Matsuda M, Yamamoto T, Ishikawa E, Kaneko MK, Yamazaki K, Kato Y, Matsumura A
  • Journal Title: Brain Tumor Pathology Volume: 28(2) Pages: 115-123
  • Peer Reviewed
• [Journal Article] Progressive adult primary glioblastoma in the medulla oblongata with an unmethylated
  • Author(s): Yoshikawa A, Nakada M, Watanabe T, Hayashi Y, Sabit H, Kato Y, Suzuki S, Ooi A, Sato H, Hamada JI
  • Journal Title: MGMT promoter and without an IDH mutation. Brain Tumor Pathol Volume: (in press)
  • DOI: DOI:10.1007/s10014-012-0118-9
  • Peer Reviewed
• [Presentation] グリオーマの予後診断マーカーとして有用な変異型IDH1/2 に対する特異的抗体の開発 (2012)
  • Author(s): 加藤幸成
  • Organizer: 第32回日本分子腫瘍マーカー研究会
  • Place of Presentation: 札幌、さっぽろ芸文館
  • Year and Date: 2012-09-18
• [Remarks] 東北大学大学院医学系研究科・加藤研究室
  • URL: http://www.med-tohoku-antibody.com/