2012 Fiscal Year Final Research Report
Comprehensive analysis of glioma LOH regions using next-generation sequencing
| Project/Area Number |
23791608
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 脳腫瘍 / 神経膠腫 / next-generation sequencing / LOH |
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| Research Abstract |
Mutation analyses on patient-derived glioma tissues: IDH1/2 mutations and LOH on 1p, 19q, 10, and 17p were analyzed on 162 patient-derived glioma tissues using conventional capillary sequencer. IDH1 mutant primary GBMs showed distinct clinical specificities. By IDH mutation and 1p/19q LOH analyses, grade III oligodendroglial tumors were divided 3 subtypes showing different clinical outcomes. Comprehensive mutation analysis on glioma cancer stem cells using next-generation sequencer: Four patient-derived GBM sphere cultures were analyzed by amplicon PCR using a Truseq Amplicon Cancer Panel, followed by next-generation sequencing using a Miseq instrument (Illumina). Bioinformatics analysis was performed using 'CLC GenomicsWorkbench' software
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Research Products
(5 results)
