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2012 Fiscal Year Final Research Report

The effects and mechanism of bone marrow stromal cells for spinalcord injury

Research Project

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Project/Area Number 23791631
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionUniversity of Fukui

Principal Investigator

NAKAJIMA Hideaki  福井大学, 医学部, 助教 (10397276)

Project Period (FY) 2011 – 2012
Keywords骨 / 脊椎脊髄病学
Research Abstract

The precise mechanisms through which transplanted MSC attenuate inflammation after SCI are still unclear. The transplanted MSC migrated within the injured spinal cord without differentiating into glial or neuronal elements. MSC transplantation was associated with marked changes in the SCI environment, with significant increases in IL-4 and IL-13 levels reductions in TNF-α and IL-6 levels. This was associated simultaneously with increased numbers of alternatively activated macrophages (M2 phenotype: arginase -1 or CD206-positive) and decreased numbers of classically activated macrophages (M1 phenotype: iNOS or CD16/32-positive). These changes were associated with functional locomotion recovery in the MSC transplanted group, which correlated with preserved axons, less scar tissue formation and increased myelin sparring. Our results suggested that acute transplantation of MSC after SCI modified the inflammatory environment by shifting the macrophage phenotype from M1 to M2, and that this may reduce the effects of the inhibitory scar tissue in the subacute/chronic phase after injury to provide a permissive environment for axonal extension and functional recovery.

  • Research Products

    (11 results)

All 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results)

  • [Journal Article] Blockade of interleukin-6 signaling inhibits the classic pathway and promotes an alternative pathway of macrophage activation after spinal cord injury in mice2012

    • Author(s)
      Guerrero AR, Uchida K, Nakajima H, Watanabe S, Nakamura M, Johnson WE, Baba H
    • Journal Title

      J Neuroinflammation

      Volume: 27(9) Pages: 40

    • Peer Reviewed
  • [Journal Article] Transplantation of mesenchymal stem cells promotes an alternative pathway of macrophage activation and functional recovery after spinal cord injury2012

    • Author(s)
      Nakajima H, Uchida K, Guerrero AR, Watanabe S, Sugita D, Takeura N, Yoshida A, Long G, Wright KT, Johnson WE, Baba H
    • Journal Title

      J Neurotrauma

      Volume: 29 Pages: 1614-1625

    • Peer Reviewed
  • [Journal Article] High-mobility Group Box-1 and Its Receptors Contribute to Proinflammatory Response in the Acute Phase of Spinal Cord Injury in Rats2011

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Hirai T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Zhu P, Baba H
    • Journal Title

      Spine

      Volume: 36 Pages: 2122-2129

    • Peer Reviewed
  • [Journal Article] Tumor necrosis factor-alpha antagonist reduces apoptosis of neurons and oligodendroglia in rat spinal cord injury2011

    • Author(s)
      Chen KB, Uchida K, Nakajima H, Yayama T, Guerrero AR, Kobayashi S, Ma WY, Liu SY, Baba H
    • Journal Title

      Spine

      Volume: 36 Pages: 1350-1358

    • Peer Reviewed
  • [Presentation] 骨髄由来間葉系幹細胞移植が脊髄損傷後急性期における macrophage 分画に与える影響2012

    • Author(s)
      中 嶋 秀 明 、内 田 研 造 、Alexander Rodriguez Guerrero、渡邉修司、杉田大輔、竹浦直人、吉田藍、馬場久敏
    • Organizer
      第27回日本整形外科学会基礎学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      20121026-27
  • [Presentation] Transplantation of mesenchymal stem cells drrived from bone marrow after spinal cord injury contribute functional recovery and tissue regeneration by promoting alternative pathway of macrophage activation2012

    • Author(s)
      Nakajima H, Uchida K, Guerrero AR, Watanabe S, Takeura N, Yoshida A, Baba H
    • Organizer
      International Society of Stem Cell Research Annual Meeting
    • Place of Presentation
      Yokohama
    • Year and Date
      20120613-16
  • [Presentation] 骨髄間質細胞移植が脊髄損傷後 macrophage の動態に与える影響2012

    • Author(s)
      中嶋秀明 、内田研造、Alexander Rodriguez Guerrero、渡邉修司、杉田大輔、竹浦直人、馬場久敏
    • Organizer
      第85回日本整形外科学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      20120517-20
  • [Presentation] 骨間質細胞移植が脊髄損傷後微小環境の変化2012

    • Author(s)
      中嶋秀明 、内田研造 、Alexander Rodriguez Guerrero、渡邉修司、杉田大輔、竹浦直人、吉田藍、馬場久敏
    • Organizer
      第41回日本脊椎脊髄病学会学術集会
    • Place of Presentation
      久留米
    • Year and Date
      20120419-21
  • [Presentation] Transplanted bone marrow stromal cells promote functional recovery, tissue regeneration withproduction of neuroprotectivemacrophage after spinal cord injury2011

    • Author(s)
      Nakajima H, Uchida K, Alexander Rodriguez G, Yayama T, Watanabe S, Baba H
    • Organizer
      The 39th CSRS Annual Meeting
    • Place of Presentation
      Arizona, USA
    • Year and Date
      20111208-10
  • [Presentation] Prevention of the development of inflammatory tissue damage and apoptosis of neurons and oligodendroglia after spinal cord injury by tumor necrosis factor-alpha antagonist2011

    • Author(s)
      Nakajima H, Uchida K, Chen KB, Hirai T, Alexander Rodriguez G, Sugita D, Baba H
    • Organizer
      The CSRS Eurpearn Section Annual Meeting
    • Place of Presentation
      Istanbul, Turkey
    • Year and Date
      20110608-11
  • [Presentation] 脊髄損傷に対する骨髄由来Mesenchymal Syem Cell移植の有用性の検討-急性期における macrophage phenotype に与える影響-2011

    • Author(s)
      中嶋秀明 、内田研造 、Alexander Rodriguez Guerrero、渡邉修司、杉田大輔、竹浦直人、吉田藍、馬場久敏
    • Organizer
      第30回日本運動器移植・再生医学研究会
    • Place of Presentation
      福岡
    • Year and Date
      2011-09-25

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Published: 2014-09-25  

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