Development of a method of enhancing antitumor drug delivery byextracellular environment control

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791634
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Mie University
• Principal Investigator: MATSUBARA Takao 三重大学, 医学部附属病院, 助教 (30422827)
• Project Period (FY): 2011 – 2012
• Keywords: 骨・軟部腫瘍学

Research Abstract

The results of this study showed that osteosarcoma, lung cancer and gastric cancer cells which exposed to the complex tumor micro-environment (i.e. high hydrostaticpressure, hypoxic, acidic and low nutrition) controlled their own cell growth and invasiveness and metastasis ability to survie in such severe tumor micro-environmental condition. On the other hand, the release of various cytokines for survive can be integrated to amplify acridine orangewhich is a photosensitizer for malignant tumors, and the contol of micro-tumor environmentenhance the cytotoxic effects by photodynamic therapy.

Research Products

• [Journal Article] Can a less radical surgery using photodynamic therapy with acridine orange be equal to a wide-margin resection? (2013)
  • Author(s): Matsubara T, Kusuzaki K, Matsumine A, Nakamura T, Sudo A
  • Journal Title: Clin Orthop Relat Res. Volume: 471(3) Pages: 792-802
  • DOI: DOI:10.1007/s11999-012-2616-9.
• [Journal Article] Translational research of photodynamic therapy with acridine orange which targets cancer acidity (2012)
  • Author(s): Kusuzaki K, Hosogi S, Ashihara E, Matsubara T, Satonaka H, Nakamura T, Matsumine A, Sudo A, Uchida A, Murata H, Baldini N, Fais S, Marunaka Y
  • Journal Title: Curr Pharm Des Volume: 18(10) Pages: 1414-20
• [Journal Article] Acridine orange inhibits pulmonary metastasis of mouse osteosarcoma (2011)
  • Author(s): Satonaka H, Kusuzaki K, Akeda K, Tsujii M, Iino T, Uemura T, Matsubara T, Nakamura T, Asanuma K, Matsumine A, Sudo A
  • Journal Title: Anticancer Res. Volume: 31(12) Pages: 4163-8
• [Presentation] Acridine Orange Therapy as a New Less-invasive Limb Salvage Surgery for Rhabdomyosarcomas and Synovial Sarcomas. (2013)
  • Author(s): Takao Matsubara, Katsuyuki Kusuzaki, Akihiko Matsumine, Kunihiro Asanuma, Tomoki Nakamura, Akihiro Sudo
  • Organizer: AAOS (American Association of Orthopaedic Surgeons) 2013 Annual Meeting
  • Place of Presentation: Chicago, IL
  • Year and Date: 20130319-24
• [Presentation] A MINIMALLY INVASIVE SURGERY FOR BONE METASTASES USING THE COMBINATION OF PHOTODYNAMIC THERAPY AND HYPERTHERMIA TREATMENT (2012)
  • Author(s): Takao Matsubara, Katsuyuki Kusuzaki, Akihiko Matsumine, Kunihiro Asanuma, Tomoki Nakamura, Atsumasa Uchida, Akihiro Sudo.
  • Organizer: CTOS (Connective Tissue Oncology Society) 17th Annual Meeting
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 20121114-17
• [Presentation] 骨肉腫細胞における細胞外環境因子とアクリジンオレンジ腫瘍内集積濃度の関係 (2012)
  • Author(s): 松原孝夫楠崎克之 松峯昭彦 浅沼邦洋 中村知樹 内田淳正 須藤啓広
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Year and Date: 20121026-27
• [Presentation] A minimally invasive surgery for bone metastases using photodynamic therapy with acridine orange (2012)
  • Author(s): Takao Matsubara, Katsuyuki Kusuzaki, Akihiko Matsumine, Kunihiro Asanuma, Tomoki Nakamura, Atsumasa Uchida, Akihiro Sudo
  • Organizer: A minimally invasive surgery for bone metastases using photodynamic therapy with acridine orange
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20121012-13
• [Presentation] Acridine orange used for photodynamic therapy accumulates in malignant tumor cells by control the tumor microenvironments. (2012)
  • Author(s): Takao Matsubara,Katsuyuki Kusuzaki, Akihiko Matsumine, Kunihiro Asanuma, Tomoki Nakamura, Atsumasa Uchida, Akihiro Sudo
  • Organizer: ISPDC (Internationl Society for Proton Dynamics in Cancer) 3rd Symposium
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20121012-13
• [Presentation] 転移性骨腫瘍に対するアクリジンオレンジ光線力学的療法および磁性体温熱療法併用治療法の検討 (2012)
  • Author(s): 松原孝夫 楠崎克之 松峯昭彦 浅沼邦洋中村知樹 内田淳正 須藤啓広
  • Organizer: 71回 日本癌学会学術集会
  • Year and Date: 20120919-21
• [Presentation] A New Therapeutic Photodynamic Surgery Using Acridine Orange for Rhabdomyosarcomas and Synovial Sarcomas. (2012)
  • Author(s): Takao Matsubara, Katsuyuki Kusuzaki, Akihiko Matsumine, Kunihiro Asanuma, Tomoki Nakamura, Atsumasa Uchida, Akihiro Sudo
  • Organizer: APMSTS (Asia Pacific Musculoskeletal Tumor Society) Meeting
  • Place of Presentation: Kuala lumpur, Malaysia
  • Year and Date: 20120906-09
• [Presentation] 緩和的治療としての転移性骨腫瘍に対するアクリジンオレンジ療法および磁性体温熱療法併用治療 (2012)
  • Author(s): 松原孝夫、楠崎克之、松峯昭彦、中村知樹、浅沼邦洋、須藤啓広
  • Organizer: 中部日本整形災害外科学会 第120回学術集会
  • Place of Presentation: 大阪
  • Year and Date: 20120406-07
• [Presentation] A new therapeutic limb salvage surgery using acridine orange in patients with bone sarcomas (2012)
  • Author(s): Takao Matsubara, Katsuyuki Kusuzaki, Akihiko Matsumine, Akihiro Sudo
  • Organizer: AAOS (American Academy of Orthopaedic Surgerons) 2012 Annual Meeting
  • Place of Presentation: San Francisco, CA
  • Year and Date: 20120207-11
• [Presentation] The Synergistic Tumor Micro-environmental Effect of Extracellular pH, pO2, and Pressure controlled Invasiveness and Survival on Human Osteosarcoma Cells (2012)
  • Author(s): Takao Matsubara, Akihiro Sudo, Gene R DiResta, John H Healey.
  • Organizer: ORS (Orthopaedic Research Society)2012 Annual Meeting
  • Place of Presentation: San Francisco, CA
  • Year and Date: 20120204-07
• [Presentation] 化学療法感受性腫瘍に対するアクリジンオレンジ療法の検討 (2012)
  • Author(s): 松原孝夫、楠崎克之、松峯昭彦、中村知樹、浅沼邦洋、須藤啓広
  • Organizer: AO治療研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-12-08
• [Presentation] Tumor Micro-environmental Effect of Extracellular pH, pO2, and Pressure controlled Invasiveness and Survival on Human Osteosarcoma Cells. (2011)
  • Author(s): Takao Matsubara, Akihiro Sudo, Gene R DiResta, John H Healey.
  • Organizer: ISPDC (Symposium of the International Society for Proton Dynamics in Cancer) 2th Annual Meeting
  • Place of Presentation: Nice, France
  • Year and Date: 20111118-19
• [Presentation] China Clinical Outcome of Acridine Orange Therapy Supported by Photodynamic Surgery and Radiodynamic Therapy for Soft Tissue Sarcomas, Compared with Conventional Limb Salvage Surgery after Wide Resection (2011)
  • Author(s): Takao Matsubara, Katsuyuki Kusuzaki, Hiroaki Murata, Akihiko Matsumine, Tomoki Nakamura, Kunihiro Asanuma, Atsumasa Uchida, Akihiro Sudo
  • Organizer: ISOLS (International Symposium on Limb Salvage) 16th Meeting
  • Place of Presentation: Beiging
  • Year and Date: 20110915-18
• [Presentation] 悪性骨軟部腫瘍切除後の骨欠損に対する術中処理自家骨移植法の治療成績 (2011)
  • Author(s): 松原孝夫 松峯昭彦 楠崎克之 中村知樹 浅沼邦洋 渥美覚 内田淳正 須藤啓広
  • Organizer: 第43回日整会骨・軟部腫瘍学会
  • Place of Presentation: 京都
  • Year and Date: 20110714-15
• [Presentation] 悪性骨軟部腫瘍における腫瘍細胞外pH と予後の検討 (2011)
  • Author(s): 松原孝夫、楠崎克之、中村知樹、浅沼邦洋、松峯昭彦、須藤啓広
  • Organizer: 第116回中部整形外科・災害外科学会
  • Place of Presentation: 高知
  • Year and Date: 20110407-08
• [Presentation] 悪性軟部腫瘍におけるアクリジンオレンジ療法至適症例の検討 (2011)
  • Author(s): 松原孝夫, 楠崎克之, 松峯昭彦, 中村知樹、浅沼邦洋, 須藤啓広
  • Organizer: 第2回AO治療研究会
  • Place of Presentation: 京都
  • Year and Date: 2011-11-05