Regeneration ofintervertebral disc using iPS cell and application of in vivo iPS cell bank.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791639
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: KAKUTANI Kenichiro 神戸大学, 医学部附属病院, 助教 (10533739)
• Project Period (FY): 2011 – 2012
• Keywords: iPS 細胞 / 椎間板再生 / 動的圧負荷 / メカノレセプター

Research Abstract

We employed the co-culture method with iPS cells and IVD cells to differentiate iPS cells. At first, we directly co-cultured iPS cells with IVD cells. We induced embryoid body from iPS cells, in addition embryoid body cells were treated with retinoic acid for seven days. After treatment, mesenchymal stem like cells were differentiated.
Furthermore these differentiated cells were co-cultured with osteoblast inducer reagent for three weeks. We have confirmed the potential of mesenchymal stem like cells to differentiate the osteoblast cell by Alizarin red staining. The authors, for the first time, demonstrated that short-term dynamic compression induced the moderate IVD degeneration. The results of current study were considered to reflect early IVD degeneration and revealed precise mechanotransduction pathways involved with intervertebral disc degeneration.

Research Products

• [Journal Article] 骨・関節のバイオメカニクス-最近の進歩】脊椎椎間板の力学的特性 動的負荷培養装置を用いた機械受容体の検討 (2012)
  • Author(s): 蔵川拓外、角谷賢一朗、森田有亮、前野耕一郎、黒坂昌弘、西田康太郎
  • Journal Title: 整形災害外科 Volume: 55 Pages: 815-819
• [Presentation] 頚椎後方再建固定術後に発生する分節性上肢麻痺のリスクファクター(頚椎後弯矯正角度の危険域についての検討) (2013)
  • Author(s): 蔵川拓外、宮本裕史、金山修一、鷲見正敏、金村在哲、宇野耕吉、西田康太郎
  • Organizer: 第42回日本脊椎脊髄病学会
  • Place of Presentation: 沖縄
  • Year and Date: 2013-04-26
• [Presentation] Kurakawa T,Kakutani K,Morita Y,Takada T,Maeno K,Yurube T,Yamamoto J,Hirata H,Miyazaki S,Doita M,Kurosaka M,Nishida K (2013)
  • Author(s): Kurakawa T,Kakutani K,Morita Y,Takada T,Maeno K,Yurube T,Yamamoto J,Hirata H,Miyazaki S,Doita M,Kurosaka M,Nishida K
  • Organizer: Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Texas,USA
  • Year and Date: 2013-01-29
• [Presentation] 動的圧迫負荷が椎間板細胞のメカノレセプター発現に及ぼした影響 (2012)
  • Author(s): 蔵川拓外、角谷賢一朗、森田有亮、前野耕一郎、由留部崇、張鐘穎、山本潤哉、平田裕亮、高田徹、黒坂昌弘、西田康太
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-27
• [Presentation] Biologic responses of rat intervertebral discs to mechanical loading using a dynamic organ culture system (2012)
  • Author(s): Kurakawa T,Kakutani K,Morita Y,Takada T,Maeno K,Yurube T, Zhang Z,Yamamoto J,Hirata H,Doita M,Kurosaka M,Nishida K
  • Organizer: International Society for the Study of the Lumbar Spine
  • Place of Presentation: Amsterdam,Netherlands
  • Year and Date: 2012-06-01
• [Presentation] 動的圧迫負荷がラット椎間板に及ぼす影響 (2012)
  • Author(s): 蔵川拓外、角谷賢一朗、森田有亮、前野耕一郎、由留部崇、張鐘穎、山本潤哉、平田裕亮、高田徹、黒坂昌弘、西田康太郎
  • Organizer: 第41回日本脊椎脊髄病学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-04-20
• [Presentation] Biologic Response of Rat Intervertebral Disc to Dynamic Compression -Analysis of Mechanoreceptors in Dynamic Organ Culture System (2012)
  • Author(s): Kurakawa T, Kakutani K, Morita Y, Takada T, Maeno K, Yurube T, Zhang Z, Yamamoto J, Hirata H, Doita M, Kurosaka M, Nishida K
  • Organizer: The 58th Annual Meeting of Orthopedics Research Society
  • Place of Presentation: San Francisco, California,USA
  • Year and Date: 2012-04-07