2012 Fiscal Year Final Research Report
The mechanisms of induction of autophagic cell death by histone deacetylase inhibitor SAHA
| Project/Area Number |
23791660
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | autophagic cell death |
|---|
| Research Abstract |
In electron microscope SAHA induced autophagosome in multidrug-resistant osteosarcoma cell. SAHA also induced autophagy related protein Beclin1 and LC3-I 、II in dose dependent manner. These facts demonstrated that SAHA induce autophagic cell death in multidrug-resistant osteosarcoma cell. Next, we investigated cell cycle population in osteosarcoma parental cell and multidrug-resistant cell after SAHA treatment. SAHA increased Sub-G1 fraction in parental cell, but G2/M fraction in multidrug-resistant cell. Therefore, we investigated the relationship between autopphagy and G2/M phase. As a result, we got new fact that SAHA induce autophagy and G2/M phase at the same time
|
