2013 Fiscal Year Final Research Report
Signaling Crosstalk between PPARgamma and BMP2 in Mesenchymal Stem Cells.
| Project/Area Number |
23791662
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
TAKADA Ichiro 慶應義塾大学, 医学部, 助教 (50361655)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 転写 / 間葉系幹細胞 / PPARγ / BMP2 |
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| Research Abstract |
Recent studies have revealed that PPARgamma's transactivation function is regulated by extracellular signals. Among these factors, BMP2 strongly induces bone formation, but the effect of BMP2 on PPARgamma function remains unclear. We examined the effect of BMP2 and PPARgamma in ST2 cells and found that PPARgamma activation affected BMP2's signaling pathway through epigenetic regulation. Although BMP2 did not interfere with PPARgamma-mediated adipogenesis, BMP2 increased mRNA expression levels of PPARgamma target genes (such as Fabp4 and Nr1h3) when cells were first treated with troglitazone (TRO). Moreover, PPARgamma activation affected BMP2 through enhancement of histone activation markers (acetylated histone H3 and trimethylated Lys4 of histone H3) on the Runx2 promoter. These results suggest that the order of treatment with BMP2 and a PPARgamma ligand is critical for adipogenesis and osteoblastogenesis switching in MSCs.
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[Journal Article] Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain2012
Author(s)
Shichita T, Hasegawa E, Kimura A, Morita R, Sakaguchi R, Takada I, Sekiya T, Ooboshi H, Kitazono T, Yanagawa T, Ishii T, Takahashi H, Mori S, Nishibori M, Kuroda K, Akira S, Miyake K, Yoshimura A
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Journal Title
Nat Med
Volume: 18(6)
Pages: 911-7
DOI
Peer Reviewed
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[Journal Article] Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis2012
Author(s)
Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N
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Journal Title
Nat Med
Volume: 18(3)
Pages: 405-12
DOI
Peer Reviewed
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[Journal Article] Histone 3 lysine 9 (H3K9) methyltransferase recruitment to the interleukin-2 (IL-2) promoter is a mechanism of suppression of IL-2 transcription by the transforming growth factor-β-Smad pathway2011
Author(s)
Wakabayashi Y, Tamiya T, Takada I, Fukaya T, Sugiyama Y, Inoue N, Kimura A, Morita R, Kashiwagi I, Takimoto T, Nomura M, Yoshimura A
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Journal Title
Histone 3 lysine 9 (H3K9) methyltransferase recruitment to the interleukin-2 (IL-2) promoter is a mechanism of suppression of IL-2 transcription by the transforming growth factor-β-Smad pathway
Volume: 286(41)
Pages: 35456-65
DOI
Peer Reviewed
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