2012 Fiscal Year Final Research Report
Molecular mechanism of potassium channel to target of volatile anesthetia
| Project/Area Number |
23791691
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Fukui |
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Principal Investigator |
MATSUKI Yuka 福井大学, 医学部, 助教 (10464083)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 麻酔学 |
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| Research Abstract |
We prepared E71A mutant of KcsA channel. Channels were reconstruct into the planar lipid bilayer membrane and single-channel current were recorded. The Popenwas plotted against pH and was fitted with the Hill equation, yielding a pKa of 5.2 and nH = 2.2. Open and closed dwell-time distributions of the E71A mutant at various pH conditions. Data were obtained at pH 4.0, pH 5.0, and pH 5.5 for the E71A mutant, respectively. The slowest component of the closed dwell-time appeared as the pH increased. Next, I tried to establish the planar lipid membrane method using a volatileanesthesia. After embedding potassium channels in the planar lipid membrane andwas subjected to a current recording potassium channels, it was completely sealed by using an acrylic plate.
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Research Products
(34 results)
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[Journal Article] デジタル処理による動脈圧波形の歪みの補正2012
Author(s)
三田建一郎, 佐藤倫祥, 清水久美子, 次田佳代, 松木悠佳, 田畑麻里, 信川 泰成, 上田雅史, 林和子,廣瀬宗孝, 重見 研司
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Journal Title
麻酔・集中治療とテクノロジー2009
Pages: 56-59
Peer Reviewed
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[Presentation] 福井大学附属病院緩和ケアチームにおける神経ブロック適応症例の検討2012
Author(s)
溝上真樹, 松木悠佳, 重見研司, 小坂浩隆, 西本武史, 片山寛次, 森川美羽,中澤雅子, 佐藤義高, 青木芳隆, 久保博子, 北山富士子, 高野智早, 小林美貴, 成瀬廣亮
Organizer
第18回福井県緩和医療研究会
Place of Presentation
福井
Year and Date
2012-11-10
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