2012 Fiscal Year Final Research Report
The drug-resistant mechanism and rapid detection of the Pseudomonas aeruginosa derived from urinary tract infection
| Project/Area Number |
23791756
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 緑膿菌 / 抗菌薬耐性 / 迅速診断法 |
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| Research Abstract |
Pseudomonas aeruginosa has some mechanisms of multi-drug resistance, including efflux pump system and amino acid mutation related to the drug resistance. We investigate the molecular biological mechanism of resistance to antibiotics, and seek for the rapid detection of the resistance. A hundred and fourteen strains of P.aeruginosa isolated from UTI patients were used in this study. Genomic DNA fromeach strain was subjected to PCR amplification of 225 bp in gyrA and 166 bp in ParC spanning QRDRs. After we performed DNA sequencing of these ampliconsand identification of mutations in the QRDRs, DHPLC was undertaken to investigate whether its results correlate the distinctive chromatogram with their DNA mutations pattern. In terms of efflux pump system in Pseudomonas aeruginosa, I examined the evaluation of the RNA frequency in the representative protein (mexB, mexC, mexE, mexY, muxA) related to efflux pump and the relationship with the drug resistance using technique of the quantitative real-time PCR.
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