Aberrant DNA methylation of the imprinted genes in the sperm of

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791799
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Tohoku University
• Principal Investigator: MIYAUCHI Naoko 東北大学, 医学(系)研究科(研究院), 技術補佐員 (60596162)
• Project Period (FY): 2011 – 2013
• Keywords: 生殖補助医療（ART） / 男性精子 / DNAメチル化 / ゲノムインプリント

Research Abstract

The parent-of-origin specific expression of the imprinted genes relies on DNA methylation during gametogenesis. We found aberrant DNA methylation of 22 imprinted loci in sperm from infertile men. We identified apporoximately 25%(84/337)abnormal methylation cases. We found strong correlation between methylation error and poor quality sperm(oligozoospermia),and low imprint rate and live-birth. We need carefully follow-up ART children.

Research Products

• [Journal Article] Stability of the abnormal imprinting of human induced pluripotent stem cells (2013)
  • Author(s): Hiura H, Toyoda M, Okae H, Sakurai M, Miyauchi N, Sato A, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Nishino K, Umezawa A, Arima T
  • Journal Title: BMC Genetics Volume: 14 Pages: 32
  • DOI: 10.1186/1471-2156-14-32
  • Peer Reviewed
• [Journal Article] DNA methylation errors in imprinting disorders and assisted reproductive technologies (2013)
  • Author(s): Chiba H, Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Arima T
  • Journal Title: Pediatrics international Volume: 55 Pages: 542-549
  • DOI: 10.1111/ped.12185
  • Peer Reviewed
• [Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method (2012)
  • Author(s): Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T
  • Journal Title: BMC Medical Genomics Volume: 5 Pages: 8-17
  • Peer Reviewed
• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproductive technologies (2012)
  • Author(s): Hiura H, Okae H, Miyauchi N, Sato F, Sato A , Van De Pette M, John R M, Kagami M, Nakai K, Soejima H, Ogata T, Arima T
  • Journal Title: Human Reproduction Volume: 27 (8) Pages: 2541-2548
  • DOI: 10.1093/humrep/des197
  • Peer Reviewed