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2013 Fiscal Year Final Research Report

Elucidation of mechanism of the hyperuricemia accompanied by pregnancy induced hypertension (PIH) and multiple pregnancy.

Research Project

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Project/Area Number 23791852
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionKyorin University

Principal Investigator

KIMURA Toru  杏林大学, 医学部, 助教 (30433725)

Project Period (FY) 2011 – 2013
Keywords胎盤 / 尿酸 / 上皮輸送 / パラセルラー輸送 / 妊娠
Research Abstract

In this study, we compared maternal serum urate levels with those of umbilical cord blood and investigated urate transport mechanisms in BeWo cells, a trophoblast-derived cell line. There were no significant differences in serum urate levels between maternal blood and umbilical cord blood, suggesting that urate is freely movable at the placenta. RT-PCR and immunohistochemistry showed that urate transporters including OAT4, OAT10, URATv1 and ABCG2 were expressed in the syncytiotrophoblast cells in the placenta as well as BeWo cells. Despite expressing urate transporters, BeWo cells did not take up urate. After confirming the formation of tight junctions of these cells cultured on the transwell, urate transport between upper and lower chambers was measured. Urate moved through BeWo cell monolayers with non-saturation kinetics and this movement was observed even when the cells were incubated at 4 degree, suggesting that urate moves through the paracellular route by simple diffusion.

  • Research Products

    (8 results)

All 2014 2013 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] Paracellular route is the major urate transport pathway across the blood-placental barrier2014

    • Author(s)
      Uehara I, Kimura T, Tanigaki S, Fukutomi T, Sakai K, Shinohara Y, Ichida K, Iwashita M, Sakurai H
    • Journal Title

      Physiol Rep

      Volume: 2(5) Pages: e12013

    • DOI

      10.14814/phy2.12013

    • Peer Reviewed
  • [Journal Article] Expression of SLC2A9 isoforms in the kidney and their localization in polarized epithelial cells2014

    • Author(s)
      Kimura T, Takahashi M, Yan K, Sakurai H
    • Journal Title

      PLoS One

      Volume: 9(1) Pages: e84996

    • DOI

      10.1371/journal.pone.0084996

    • Peer Reviewed
  • [Journal Article] 妊娠時における母体・胎児間の尿酸代謝解析2013

    • Author(s)
      木村徹、上原一朗、谷垣伸治、岩下光利、安西尚彦、櫻井裕之
    • Journal Title

      発達腎研究会誌

      Volume: 21 Pages: 7-11

  • [Presentation] 上皮細胞における尿酸のparacellular輸送とclaudinの発現2013

    • Author(s)
      木村徹、塚田愛、大槻純男、福冨俊之、市田公美、櫻井裕之
    • Organizer
      第8回トランスポーター研究会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      20130615-16
  • [Presentation] 上皮細胞における尿酸輸送とclaudinの発現2013

    • Author(s)
      木村徹、塚田愛、大槻純男、福冨俊之、市田公美、櫻井裕之
    • Organizer
      日本薬学会第133年会
    • Place of Presentation
      横浜
    • Year and Date
      20130327-30
  • [Presentation] 上皮細胞における尿酸のparacellular輸送2013

    • Author(s)
      木村徹、塚田愛、市田公美、櫻井裕之
    • Organizer
      第46回日本痛風・核酸代謝学会総会
    • Place of Presentation
      新宿
    • Year and Date
      20130213-15
  • [Presentation] Uric acid crosses the placental barrier through paracellular route2012

    • Author(s)
      Toru Kimura, Ichiro Uehara, Shinji Tanigaki, Mitsutoshi Iwashita, Hiroyuki Sakurai
    • Organizer
      2012 ASCB Annual Meeting
    • Place of Presentation
      San Francisco, CA, USA
    • Year and Date
      20121215-19
  • [Presentation] Urate transport between maternal and umbilical cord blood in the placent2011

    • Author(s)
      木村徹、上原一郎、谷垣伸治、岩下光利、安西尚彦、櫻井裕之
    • Organizer
      International Joint Meeting of Cellular and Molecular Physiology in Epithelia
    • Place of Presentation
      北里大学(白金キャンパス)
    • Year and Date
      2011-07-31

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Published: 2015-06-25  

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