Identification of hypo-pharyngeal and esophageal squamous cell carcinoma molecular pathways based on microRNA expression signature

2012 Fiscal Year Final Research Report

• Project/Area Number: 23791876
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Chiba University
• Principal Investigator: KIKKAWA Naoko 千葉大学, 医学部附属病院, 医員 (50400924)
• Project Period (FY): 2011 – 2012
• Keywords: (1)頭頸部癌 / (2)マイクロRNA

Research Abstract

MicroRNAs (miRNAs) are a class of small non-coding cRNA molecules of 19-22 nucleotides that regulate protein-coding gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. A growing body of evidence suggests that miRNAs are aberrantly expressed in several types of human cancer, and that they play significant roles in the initiation, progression and metastasis of human cancers. The results of the miRNA expression signatures of hypo-pharyngeal and esophageal squamous cell carcinoma indicated that miR-218 was downregulated in cancer cells and suggested as a tumor suppressive miRNA. Gain-of-function assays demonstrated that miR-218 inhibited cancer cell proliferation and invasion through targeting LAMB3. The LAMB3 are an important and biologically active part of the basal lamina, influencing cell differentiation, migration,adhesion, proliferation, and survival.

Research Products

• [Journal Article] Tumor suppressive microRNA-218 inhibits cancer cell migration and invasion through targeting laminin-332 in head and necksquamous cell carcinoma (2012)
  • Author(s): Kinoshita T, Hanazawa T, Nohata N,Kikkawa N, Enokida H, Yoshino H, Yamasaki T, Hidaka H, Nakagawa M, Okamoto Y, Seki N
  • Journal Title: Oncotarget Volume: 3(11) Pages: 1386-400
  • Peer Reviewed
• [Journal Article] Tumour suppressive microRNA-874 regulates novel cancer networks in maxillary sinussquamous cell carcinoma (2011)
  • Author(s): Nohata N, Hanazawa T, Kikkawa N, Sakurai D, Fujimura L, Chiyomaru T, Kawakami K, Yoshino H, Enokida H, Nakagawa M, Katayama A, Harabuchi Y, Okamoto Y, Seki N
  • Journal Title: Br J Cancer Volume: 105 Pages: 833-41
  • Peer Reviewed
• [Journal Article] (2010) miR-489 is a tumour-suppressive miRNA target PTPN11 in hypopharyngeal squamous cell carcinoma (HSCC) (2010)
  • Author(s): Kikkawa N, Hanazawa T, Fujimura L, Nohata N, Suzuki H, Chazono H, Sakurai D,Horiguchi S, Okamoto Y, Seki N
  • Journal Title: Br J Cancer Volume: 103 Pages: 877-84
  • Peer Reviewed
• [Presentation] Yoshitaka Okamoto, Naohiko SekiTumor suppressive microRNA-375 regulates molecular targets in head and neck squamous cell carcinoma (2012)
  • Author(s): Naoko Kikkawa, Takashi Kinosita, NijiroNohata, Toyoyuki Hanazawa, Hirofumi Yosino, Hedeki Enokida, Masayuki Nakagawa
  • Organizer: 第71回日本癌学会
  • Place of Presentation: ロイトン札幌 札幌
  • Year and Date: 20120919-20
• [Presentation] 関直彦癌抑制型microRNA-375が制御する頭頸部扁平上皮癌の新規分子ネットワークの解明 (2012)
  • Author(s): 吉川直子、花澤豊行、野畑二次郎、木下崇、鈴木誉、茶薗英明、櫻井大樹、岡本美孝
  • Organizer: 第36回日本頭頸部癌学会学術講演会
  • Place of Presentation: 日島根県民会館 島根
  • Year and Date: 20120607-08
• [Presentation] 関直彦頭頸部扁平上皮癌におけるmicroRNA-1の癌抑制機能と新規分子ネットワークの解明 (2011)
  • Author(s): 吉川直子、花澤豊行、野畑二次郎、ムラディルムラリフ、鈴木誉、茶薗英明、櫻井大樹、佐々木慶太、岡本美孝
  • Organizer: 第35回日本頭頸部癌学会
  • Place of Presentation: ウインクあいち 名古屋
  • Year and Date: 20110609-10
• [Presentation] 岡本美孝下咽頭癌における癌抑制マイクロRNAの探索 (2011)
  • Author(s): 吉川直子、花澤豊行、野畑二次郎、鈴木誉、茶薗英明、櫻井大樹、堀口茂俊
  • Organizer: 第112回日本耳鼻咽喉科学会
  • Place of Presentation: 国立京都国際会館 京都
  • Year and Date: 20110519-20-21
• [Remarks]
  • URL: http://genome.jet.jp